Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,912,061	T→C	E536E (GAA→GAG)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,912,061	0	T	C	100.0%	183.2 / NA	53	E536E (GAA→GAG)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/21);  total (32/21)

GATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAAT  >  NC_000913/3911983‑3912138                                                                               |                                                                              gatgTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAAcctc                                                                             >  2:777029/1‑81 (MQ=255) gatgTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAAcctc                                                                             >  1:777016/1‑81 (MQ=255)   tgtgCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAAcctctt                                                                           <  1:776974/81‑1 (MQ=255)    gtgCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAAcctctt                                                                           >  1:777040/1‑80 (MQ=255)     tgCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACctcttc                                                                          <  1:776975/80‑1 (MQ=255)       cATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAAt                                                                       <  1:776976/81‑1 (MQ=255)          gTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGtt                                                                    >  1:777024/1‑81 (MQ=255)           ttATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGtt                                                                    <  2:776977/80‑1 (MQ=255)            tATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTg                                                                   >  2:777027/1‑80 (MQ=255)              tCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGAt                                                                >  1:777060/1‑81 (MQ=255)               cGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGAtt                                                               >  2:777054/1‑81 (MQ=255)                gCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGAttt                                                              >  2:777033/1‑81 (MQ=255)                gCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGAtt                                                               <  2:776978/80‑1 (MQ=255)                 cTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGAttt                                                              >  1:777034/1‑80 (MQ=255)                    cTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGt                                                          >  1:777025/1‑81 (MQ=255)                    cTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGt                                                          <  2:776979/81‑1 (MQ=255)                         aaaaCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTc                                                     <  1:776980/81‑1 (MQ=255)                          aaaCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTc                                                     >  1:777039/1‑80 (MQ=255)                          aaaCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTc                                                     >  1:777063/1‑80 (MQ=255)                             cccGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGc                                                 <  1:776981/81‑1 (MQ=255)                              ccGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCa                                                <  1:776982/81‑1 (MQ=255)                                gCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAAt                                              >  2:777026/1‑81 (MQ=255)                                gCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAAt                                              >  2:777062/1‑81 (MQ=255)                                 cATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAAtt                                             >  1:777065/1‑81 (MQ=255)                                 cATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAAtt                                             >  1:777045/1‑81 (MQ=255)                                  aTCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTc                                            <  1:776983/81‑1 (MQ=255)                                     cTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCgtt                                         <  1:776987/81‑1 (MQ=255)                                     cTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCgtt                                         <  1:776985/81‑1 (MQ=255)                                     cTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCgtt                                         <  2:776986/81‑1 (MQ=255)                                     cTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCgt                                          <  2:776984/80‑1 (MQ=255)                                           ggCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCgg                                   <  2:776988/81‑1 (MQ=255)                                           ggCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCg                                    <  2:776989/80‑1 (MQ=255)                                            gCGAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGt                                  <  1:776990/81‑1 (MQ=255)                                              gAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTg                                 >  2:777041/1‑80 (MQ=255)                                              gAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTg                                 >  2:777038/1‑80 (MQ=255)                                              gAAGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTg                                 >  1:777056/1‑80 (MQ=255)                                               aaGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGca                               <  2:776991/81‑1 (MQ=255)                                               aaGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGc                                >  2:777068/1‑80 (MQ=255)                                                aGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTTAGTTTTTCCAGCAATTCGTTGTTCGGTGcaa                              >  2:777037/1‑81 (MQ=255)                                                aGACATACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaa                              >  1:777057/1‑81 (MQ=255)                                                    aTACAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaac                          <  2:776992/81‑1 (MQ=255)                                                      aCAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaacaa                        >  2:777047/1‑81 (MQ=255)                                                      aCAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaacaa                        >  1:777058/1‑81 (MQ=255)                                                       cAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaacaa                        >  1:777035/1‑80 (MQ=255)                                                       cAACTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaacaa                        >  2:777032/1‑80 (MQ=255)                                                          cTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAAc                    <  2:776993/81‑1 (MQ=255)                                                          cTGACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAAc                    >  2:777055/1‑81 (MQ=255)                                                            gACCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCg                  >  2:777069/1‑81 (MQ=255)                                                             aCCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAAcc                   <  2:776994/79‑1 (MQ=255)                                                             aCCGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCgg                 >  1:777067/1‑81 (MQ=255)                                                              ccGCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTCTTCGGTGCAACAACAATAACCgg                 >  2:777098/1‑80 (MQ=255)                                                               cGCCACGCGCGCGAACCTCTTCAATGTTGGAATTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCa               <  2:776995/81‑1 (MQ=255)                                                                gCCACGCGCTCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCa               >  1:777090/1‑80 (MQ=255)                                                                gCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCa               >  1:777064/1‑80 (MQ=255)                                                                gCCACGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCa               >  1:777051/1‑80 (MQ=255)                                                                   aCGCGCGCGAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATc           >  2:777052/1‑81 (MQ=255)                                                                         gcgAACCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCAt      >  2:777044/1‑80 (MQ=255)                                                                            aaCCTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAAt  <  2:776996/81‑1 (MQ=255)                                                                               |                                                                              GATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAAT  >  NC_000913/3911983‑3912138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑