Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,370	G→T	V19F (GTT→TTT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,370	0	G	T	100.0%	97.1 / NA	28	V19F (GTT→TTT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/14);  total (14/14)

TTTCAACGAAAGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTT  >  NC_000913/3931296‑3931447                                                                           |                                                                              tttCAACGAAAGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCt                                                                         <  1:782652/81‑1 (MQ=255)   tCAACGAAAGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTAc                                                                       >  1:782686/1‑81 (MQ=255)       cGAAAGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCg                                                                   <  1:782653/81‑1 (MQ=255)           aGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGatat                                                               <  2:782654/81‑1 (MQ=255)            gACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATAtt                                                              <  2:782655/81‑1 (MQ=255)                 gTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTa                                                          <  2:782656/80‑1 (MQ=255)                  tCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTAcc                                                        <  1:782657/81‑1 (MQ=255)                  tCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTAc                                                         >  1:782698/1‑80 (MQ=255)                      tGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGcc                                                    <  2:782658/81‑1 (MQ=255)                                    aaGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACtt                                      >  2:782692/1‑81 (MQ=255)                                    aaGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACtt                                      >  1:782694/1‑81 (MQ=255)                                       cAATCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGt                                   <  1:782659/81‑1 (MQ=255)                                          tCATTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGaa                                <  1:782660/81‑1 (MQ=255)                                            aTTGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATg                              >  1:782700/1‑81 (MQ=255)                                              tGCCCGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGtt                            >  1:782693/1‑81 (MQ=255)                                                 ccGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGt                         <  2:782661/81‑1 (MQ=255)                                                 ccGCGATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGt                         >  2:782695/1‑81 (MQ=255)                                                    cgATTACCCTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCg                      >  1:782704/1‑81 (MQ=255)                                                           ccTCGCGGCGATTGGATTTGTCTACGGCGATGTTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGtt               <  2:782662/81‑1 (MQ=255)                                                            cTCGCGGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGttt              <  2:782663/81‑1 (MQ=255)                                                              cgcgGCGATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTgg            >  2:782683/1‑81 (MQ=255)                                                                 ggcgATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGttt         <  2:782664/81‑1 (MQ=255)                                                                 ggcgATTGGATCTGTCTACGGCGATACTGGTACCAGCCCGTTATATACACTTCGTGTATGTTTGTCCGGCCAGTTTGGttt         >  2:782707/1‑81 (MQ=255)                                                                  gcgATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGtttt        >  1:782709/1‑81 (MQ=255)                                                                  gcgATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGtttt        >  2:782696/1‑81 (MQ=255)                                                                  gcgATTGGATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGtttt        >  2:782715/1‑81 (MQ=255)                                                                        ggATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGtt  >  2:782697/1‑81 (MQ=255)                                                                        ggATTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGt   <  1:782665/80‑1 (MQ=255)                                                                           |                                                                              TTTCAACGAAAGACTAGTCTATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTT  >  NC_000913/3931296‑3931447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑