Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,964,541	A→G	Y364H (TAT→CAT)	rhlB ←	ATP‑dependent RNA helicase RhlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,964,541	0	A	G	100.0%	165.5 / NA	47	Y364H (TAT→CAT)	rhlB	ATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/15);  total (32/15)

TCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTT  >  NC_000913/3964463‑3964618                                                                               |                                                                              tcaATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGct                                                                             >  1:792023/1‑81 (MQ=255) tcaATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGct                                                                             >  1:792008/1‑81 (MQ=255)   aaTGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGctct                                                                           >  2:791995/1‑81 (MQ=255)    aTGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCtt                                                                          >  1:792029/1‑81 (MQ=255)     tGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTc                                                                         <  2:791941/81‑1 (MQ=255)        gTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTcaca                                                                      <  2:791942/81‑1 (MQ=255)         tCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCacac                                                                     >  1:792011/1‑81 (MQ=255)          ccGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACg                                                                    >  2:792012/1‑81 (MQ=255)            ggATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGc                                                                   >  2:791985/1‑80 (MQ=255)            ggATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGc                                                                   >  1:791989/1‑80 (MQ=255)               ttGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAgg                                                               >  1:791990/1‑81 (MQ=255)                tGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGc                                                              >  1:792016/1‑81 (MQ=255)                 gTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCt                                                             >  1:792004/1‑81 (MQ=255)                   aTTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGa                                                           >  2:792009/1‑81 (MQ=255)                       gCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATa                                                         >  2:792026/1‑79 (MQ=255)                           aCCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGt                                                     <  2:791943/79‑1 (MQ=255)                           aCCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTg                                                    <  2:791944/80‑1 (MQ=255)                            ccGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGAc                                                  >  1:792001/1‑81 (MQ=255)                             cGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGAcc                                                 <  2:791945/81‑1 (MQ=255)                              ggAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCg                                                >  2:792000/1‑81 (MQ=255)                                   tGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTgc                                           >  1:792020/1‑81 (MQ=255)                                    gAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTgcg                                          >  1:792036/1‑81 (MQ=255)                                    gAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTgc                                           <  1:791946/80‑1 (MQ=255)                                     aGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTgcgc                                         >  1:792015/1‑81 (MQ=255)                                       tGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCt                                       >  1:792007/1‑81 (MQ=255)                                        gACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTg                                      >  2:792014/1‑81 (MQ=255)                                          ccAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTgcg                                    >  2:792005/1‑81 (MQ=255)                                           cAATATAGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTgcgc                                   >  2:792017/1‑81 (MQ=255)                                              tataGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGAc                                <  1:791947/81‑1 (MQ=255)                                                taGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGAcc                               <  2:791949/80‑1 (MQ=255)                                                taGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGAcc                               <  2:791948/80‑1 (MQ=255)                                                 aGGTCTCAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTg                             >  2:791994/1‑81 (MQ=255)                                                    tctcAATAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTAc                          >  2:792030/1‑81 (MQ=255)                                                        aaTAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGAc                       >  1:792019/1‑80 (MQ=255)                                                        aaTAGCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGAc                       >  2:792010/1‑80 (MQ=255)                                                            gCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAAt                   <  2:791950/80‑1 (MQ=255)                                                            gCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAAt                   <  1:791951/80‑1 (MQ=255)                                                            gCAGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATa                  >  1:792034/1‑81 (MQ=255)                                                              aGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACg                <  2:791952/81‑1 (MQ=255)                                                              aGGCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACg                >  2:792028/1‑81 (MQ=255)                                                               ggCAAATTCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACgg               >  2:792013/1‑81 (MQ=255)                                                                     ttCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACg         <  1:791953/81‑1 (MQ=255)                                                                     ttCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACg         >  2:792031/1‑81 (MQ=255)                                                                      tCAATGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACg         >  2:792046/1‑80 (MQ=255)                                                                        aaTGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTa       <  1:791954/80‑1 (MQ=255)                                                                        aaTGCATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTa       <  1:791955/80‑1 (MQ=255)                                                                         atgcatgcTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAAt     >  2:792033/1‑81 (MQ=255)                                                                            cATGCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCtt  >  2:792049/1‑81 (MQ=255)                                                                               |                                                                              TCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTT  >  NC_000913/3964463‑3964618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑