Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,188	T→C	L2L (TTA→TTG)	yigG ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,188	0	T	C	100.0%	65.9 / NA	20	L2L (TTA→TTG)	yigG	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

TTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGG  >  NC_000913/4003117‑4003260                                                                        |                                                                         tttATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAAt                                                                 <  1:802722/81‑1 (MQ=255)     ttaAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAAtttt                                                              <  1:802724/80‑1 (MQ=255)     ttaAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAAtttt                                                              <  2:802723/80‑1 (MQ=255)         aataaatGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATc                                                         >  1:802736/1‑81 (MQ=255)          ataaatGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACAtac                                                        <  1:802725/81‑3 (MQ=255)             aatGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTg                                                     >  1:802732/1‑81 (MQ=255)              atGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACACCCTTg                                                     >  1:802747/1‑80 (MQ=255)                 aaaaTATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTAt                                                  >  1:802743/1‑80 (MQ=255)                       tataACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGtctc                                           >  1:802737/1‑81 (MQ=255)                           acgacgTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGt                                       >  1:802735/1‑81 (MQ=255)                               cgTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAg                                    >  1:802739/1‑80 (MQ=255)                               cgTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGc                                   <  2:802726/81‑1 (MQ=255)                                                   aGATGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCActct                >  2:802742/1‑80 (MQ=255)                                                      tGTTGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATg            <  2:802727/81‑1 (MQ=255)                                                        ttGGGATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGc           <  2:802728/80‑1 (MQ=255)                                                          gggATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCaa         <  1:802729/80‑1 (MQ=255)                                                            gATAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAagag      >  2:802762/1‑81 (MQ=255)                                                             aTAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGt     >  1:802740/1‑81 (MQ=255)                                                              tAAATATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTc    <  1:802730/81‑1 (MQ=255)                                                                aaTATACGCAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCgg  >  2:802741/1‑81 (MQ=255)                                                                        |                                                                         TTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGG  >  NC_000913/4003117‑4003260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑