Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,964	A→G	N109S (AAC→AGC)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,964	0	A	G	100.0%	123.5 / NA	36	N109S (AAC→AGC)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/19);  total (17/19)

CAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAACGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAATTTT  >  NC_000913/396888‑397041                                                                             |                                                                              cAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGcc                                                                           <  2:89698/81‑1 (MQ=255)  aaTACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCt                                                                          <  1:89699/81‑1 (MQ=255)  aaTACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCt                                                                          >  1:89765/1‑81 (MQ=255)    tACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTgg                                                                        <  1:89700/81‑1 (MQ=255)      cTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGt                                                                       <  2:89701/80‑1 (MQ=255)         gTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTAt                                                                     <  1:89703/79‑1 (MQ=255)         gTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTAt                                                                     <  1:89704/79‑1 (MQ=255)         gTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATg                                                                    <  2:89702/80‑1 (MQ=255)           cAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATgcgc                                                                 >  1:89737/1‑81 (MQ=255)               aCTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGtt                                                             >  1:89753/1‑81 (MQ=255)                cTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTc                                                            >  2:89742/1‑81 (MQ=255)                   ggTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCt                                                           <  1:89705/79‑1 (MQ=255)                           aCTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGAt                                                 >  1:89758/1‑81 (MQ=255)                           aCTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGAt                                                 <  1:89706/81‑1 (MQ=255)                             tGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGAtt                                                >  1:89760/1‑80 (MQ=255)                               atcatcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTc                                               <  1:89707/79‑1 (MQ=255)                               atcatcTTCGTCACCTGGTTTTT‑GTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCaaa                                            >  1:89785/1‑81 (MQ=255)                                tcatcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCTTGGTATGCGCCGTTCTATGATCTGATTCaaa                                            >  2:89747/1‑81 (MQ=255)                                    cTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACcgc                                        >  1:89744/1‑81 (MQ=255)                                           aCCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAg                                  <  2:89708/80‑1 (MQ=255)                                            ccTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGtt                                <  2:89709/81‑1 (MQ=255)                                             cTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTc                               <  1:89711/81‑1 (MQ=255)                                             cTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTc                               <  1:89710/81‑1 (MQ=255)                                               ggTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTcgc                             >  2:89768/1‑81 (MQ=255)                                                gTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTcgcc                            >  1:89752/1‑81 (MQ=255)                                                gTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTcg                              <  2:89712/79‑1 (MQ=255)                                                   tttGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCa                         >  1:89767/1‑81 (MQ=255)                                                       gtggAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATaa                      <  2:89713/80‑1 (MQ=255)                                                         gcaaGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGt                   >  2:89770/3‑81 (MQ=255)                                                            aGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCAc                >  1:89782/1‑81 (MQ=255)                                                            aGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCAc                <  2:89714/81‑1 (MQ=255)                                                               cGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCAt             >  2:89757/1‑81 (MQ=255)                                                                ggggTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCAt             <  2:89715/80‑1 (MQ=255)                                                                ggggTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATc            >  2:89771/1‑81 (MQ=255)                                                                     cGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAAc        >  1:89750/1‑80 (MQ=255)                                                                          tCAGCGCCTAGTATGCGCCGTTCTATGATCTAATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAAtttt  <  2:89716/81‑1 (MQ=255)                                                                             |                                                                              CAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAACGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAATTTT  >  NC_000913/396888‑397041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑