Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,952	A→G	K66E (AAA→GAA)	rhtC →	L‑threonine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,952	0	A	G	100.0%	121.1 / NA	35	K66E (AAA→GAA)	rhtC	L‑threonine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/12);  total (23/12)

GCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGT  >  NC_000913/4007878‑4008029                                                                           |                                                                              gCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATgg                                                                         <  2:803932/81‑1 (MQ=255)  cGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGc                                                                        <  2:803933/81‑1 (MQ=255)  cGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGc                                                                        >  1:803975/1‑81 (MQ=255)     gCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTg                                                                     >  2:803981/1‑81 (MQ=255)      cTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTgg                                                                    >  1:803978/1‑81 (MQ=255)        gggCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCt                                                                  >  2:803995/1‑81 (MQ=255)         ggCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTg                                                                 <  1:803934/81‑1 (MQ=255)          gCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTg                                                                 >  2:803986/1‑80 (MQ=255)          gCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTg                                                                 >  2:803993/1‑80 (MQ=255)           cATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCa                                                               >  2:803992/1‑81 (MQ=255)           cATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCa                                                               >  1:803969/1‑81 (MQ=255)             ttACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATa                                                             <  1:803935/81‑1 (MQ=255)                    cggcgTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTg                                                        <  1:803936/79‑1 (MQ=255)                        gTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtg                                                  >  1:803983/1‑81 (MQ=255)                        gTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtg                                                  >  1:804007/1‑81 (MQ=255)                           aTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggtg                                               >  2:803987/1‑81 (MQ=255)                           aTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggtg                                               >  2:803979/1‑81 (MQ=255)                               tttGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCg                                           >  1:804005/1‑81 (MQ=255)                                  gggCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTg                                        >  1:803988/1‑81 (MQ=255)                                   ggCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTgg                                       >  1:803997/1‑81 (MQ=255)                                        ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTg                                   >  2:804000/1‑80 (MQ=255)                                        ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTg                                   <  1:803937/80‑1 (MQ=255)                                             gcgcTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtctc                             >  1:803974/1‑81 (MQ=255)                                              cgcTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATctct                            <  2:803938/81‑1 (MQ=255)                                              cgcTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATctct                            >  2:803999/1‑81 (MQ=255)                                                ctgctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgc                          <  2:803940/81‑1 (MQ=255)                                                ctgctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgc                          <  1:803939/81‑1 (MQ=255)                                                   ctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                        >  1:803998/1‑80 (MQ=255)                                                        ccTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATggg                  >  1:804009/1‑81 (MQ=255)                                                          tGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGt                 >  2:803994/1‑80 (MQ=255)                                                                 gATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGAt         >  1:804002/1‑81 (MQ=255)                                                                   ttATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGc       <  1:803941/81‑1 (MQ=255)                                                                    tATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGc       >  2:804013/1‑80 (MQ=255)                                                                      tCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc    <  2:803943/81‑1 (MQ=255)                                                                      tCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAc    <  1:803942/81‑1 (MQ=255)                                                                        gaagaaATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgt  >  2:804011/1‑81 (MQ=255)                                                                           |                                                                              GCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGT  >  NC_000913/4007878‑4008029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑