Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,033,626	A→G	Y161C (TAT→TGT)	trkH →	K(+) transporter TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,033,626	0	A	G	100.0%	167.9 / NA	48	Y161C (TAT→TGT)	trkH	K(+) transporter TrkH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

AATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGT  >  NC_000913/4033548‑4033700                                                                               |                                                                           aaTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTc                                                                          <  1:811455/81‑1 (MQ=255)  aTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTcg                                                                         >  1:811503/1‑81 (MQ=255)   tGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTcgc                                                                        >  2:811501/1‑81 (MQ=255)   tGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTcgc                                                                        >  1:811519/1‑81 (MQ=255)    ggTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTcgcg                                                                       >  2:811500/1‑81 (MQ=255)        tGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAg                                                                    <  1:811456/80‑1 (MQ=255)          gCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGaa                                                                  <  1:811457/80‑1 (MQ=255)          gCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGaa                                                                  <  1:811459/80‑1 (MQ=255)          gCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGaa                                                                  <  2:811458/80‑1 (MQ=255)               aTGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATgccc                                                            >  1:811506/1‑81 (MQ=255)                 ggggATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATgcccgg                                                          >  2:811511/1‑81 (MQ=255)                  gggATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATgcccggc                                                         <  1:811460/81‑1 (MQ=255)                        atcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCt                                                    <  1:811461/80‑1 (MQ=255)                         tcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGa                                                  <  2:811462/81‑1 (MQ=255)                         tcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGa                                                  <  2:811463/81‑1 (MQ=255)                           gtgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGaa                                                 <  1:811464/80‑1 (MQ=255)                           gtgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGa                                                  >  2:811510/1‑79 (MQ=255)                             gtTGGCGGTTGCGATACTGCATATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGa                                                  >  2:811499/1‑77 (MQ=255)                               tGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGAt                                             >  2:811515/1‑80 (MQ=255)                                ggCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATaa                                           >  2:811513/1‑81 (MQ=255)                                 gCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAAc                                          <  2:811465/81‑1 (MQ=255)                                    gTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaaa                                       <  1:811466/81‑1 (MQ=255)                                       gCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATg                                    >  2:811505/1‑81 (MQ=255)                                        cGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATgc                                   >  1:811535/1‑81 (MQ=255)                                          aTACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATgcgc                                 >  2:811526/1‑81 (MQ=255)                                             cTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCcg                              <  1:811467/81‑1 (MQ=255)                                                 cTATCCTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGaa                          <  2:811468/81‑1 (MQ=255)                                                     ccTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                      >  2:811532/1‑81 (MQ=255)                                                     ccTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                      >  2:811520/1‑81 (MQ=255)                                                      cTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                      <  1:811469/80‑1 (MQ=255)                                                      cTCGGCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCg                     >  2:811543/1‑81 (MQ=255)                                                         ggCGTGGGTGGGATGCAGCTCTGTGGCGCAGAAATGCCAGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGa                   <  1:811470/80‑1 (MQ=255)                                                         ggCGTGGGTGGGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGa                   >  1:811522/1‑80 (MQ=255)                                                            gtgggtggGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACg               <  1:811471/81‑1 (MQ=255)                                                            gtgggtggGATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACg               >  1:811529/1‑81 (MQ=255)                                                             tgggtgggATGCAGCTCTGTCGCGCAGAATTGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACgg              <  2:811473/81‑1 (MQ=255)                                                             tgggtgggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACgg              <  1:811472/81‑1 (MQ=255)                                                             tgggtgggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACgg              >  1:811527/1‑81 (MQ=255)                                                               ggtgggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGc             >  1:811534/1‑80 (MQ=255)                                                                gtgggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCg            >  2:811508/1‑80 (MQ=255)                                                                 tgggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaa          <  2:811474/81‑1 (MQ=255)                                                                  gggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaa          >  1:811544/1‑80 (MQ=255)                                                                  gggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaa          >  2:811542/1‑80 (MQ=255)                                                                   ggATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaaa         <  1:811475/80‑1 (MQ=255)                                                                    gATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAc       >  2:811518/1‑81 (MQ=255)                                                                    gATGCAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAc       >  2:811528/1‑81 (MQ=255)                                                                        cAGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCt    <  2:811476/80‑1 (MQ=255)                                                                         aGCTCTGTCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCtgt  >  2:811523/1‑81 (MQ=255)                                                                               |                                                                           AATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGT  >  NC_000913/4033548‑4033700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑