Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,064,998	C→T	A257T (GCG→ACG)	yihO ←	putative sulfoquinovose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,064,998	0	C	T	100.0%	95.9 / NA	27	A257T (GCG→ACG)	yihO	putative sulfoquinovose transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

ATACTGAAGAATCCCATCCACGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGCCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGAAATATC  >  NC_000913/4064925‑4065074                                                                          |                                                                             aTACTGAAGAATCCCATCCACGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGttt                                                                        >  2:819536/1‑80 (MQ=255)               cATCCACGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATgccgc                                                         <  1:819494/81‑1 (MQ=255)                  ccACGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAg                                                      >  2:819528/1‑81 (MQ=255)                    aCGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAggg                                                    >  1:819535/1‑81 (MQ=255)                      gACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTa                                                  <  1:819495/81‑1 (MQ=255)                           tAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTacac                                               >  2:819532/1‑79 (MQ=255)                                 aaTATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAg                                        <  2:819496/80‑1 (MQ=255)                                  atatCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGACATTAAATGCCGCCAGGGTACACAAGTTAGc                                       >  2:819541/1‑80 (MQ=255)                                    atCATTCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAAt                                    >  2:819526/1‑81 (MQ=255)                                        ttCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCa                                 >  1:819529/1‑80 (MQ=255)                                        ttCAGCACATACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCa                                 >  1:819549/1‑80 (MQ=255)                                              acaTACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATTCATAAAAcc                          <  2:819497/81‑1 (MQ=255)                                                aTACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAAcc                          <  2:819498/79‑1 (MQ=255)                                                aTACTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAg                        >  1:819522/1‑81 (MQ=255)                                                  aCTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGc                       >  1:819562/1‑80 (MQ=255)                                                   cTGGGTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCa                      <  2:819499/80‑1 (MQ=255)                                                      ggTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAAt                    <  1:819501/79‑1 (MQ=255)                                                      ggTGTAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATgg                  <  2:819500/81‑1 (MQ=255)                                                       gtgtAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGc                 <  1:819503/81‑1 (MQ=255)                                                       gtgtAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGc                 <  1:819502/81‑1 (MQ=255)                                                         gtAATAGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCg                >  2:819540/1‑80 (MQ=255)                                                              aGACCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTcc          >  1:819539/1‑81 (MQ=255)                                                                aCCTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCg         >  2:819548/1‑80 (MQ=255)                                                                  cTGAATCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGaaa      >  2:819534/1‑81 (MQ=255)                                                                     aaTCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGAAat     <  1:819504/79‑1 (MQ=255)                                                                      aTCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGAAATATc  <  2:819506/81‑1 (MQ=255)                                                                      aTCGTCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGAAATATc  <  1:819505/81‑1 (MQ=255)                                                                          |                                                                             ATACTGAAGAATCCCATCCACGACAATAAATTAATATCATTCAGCACATACTGGGTGTAATAGACCTGAATCGCCAGTTTGATATTAAATGCCGCCAGGGTACACAAGTTAGCAATGCATAAAACCAGCAATGGCGGATTCCGAAATATC  >  NC_000913/4064925‑4065074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑