| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,076,654 | C→T | A170V (GCG→GTG) | yihY → | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,076,654 | 0 | C | T | 98.1% | 182.3 / ‑5.6 | 53 | A170V (GCG→GTG) | yihY | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (28/24); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGC > NC_000913/4076577‑4076731 | gatgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgag > 1:822725/1‑81 (MQ=255) atgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgagc < 2:822651/81‑1 (MQ=255) tgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgagcg < 1:822653/81‑1 (MQ=255) tgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgagcg < 1:822652/81‑1 (MQ=255) gatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgagcga > 2:822738/1‑81 (MQ=255) atTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgtgagcga < 1:822655/80‑1 (MQ=255) atTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGAt < 2:822654/81‑1 (MQ=255) atTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGAt < 2:822656/81‑1 (MQ=255) ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGAtc < 2:822657/81‑1 (MQ=255) ttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGAtct < 2:822658/80‑1 (MQ=255) aaCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCaa > 1:822749/1‑81 (MQ=255) aaCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCa < 2:822659/80‑1 (MQ=255) aCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCaa > 1:822735/1‑80 (MQ=255) aCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAAt < 1:822660/81‑1 (MQ=255) gCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATAc > 1:822741/1‑81 (MQ=255) cTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACt < 1:822661/81‑1 (MQ=255) tGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTg > 2:822758/1‑81 (MQ=255) ggggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGt > 1:822732/1‑81 (MQ=255) gggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTc < 2:822662/81‑1 (MQ=255) ggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCa < 2:822663/81‑1 (MQ=255) ccGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATc < 2:822664/81‑1 (MQ=255) cGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCg < 1:822665/81‑1 (MQ=255) gctgctGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGa > 1:822747/1‑81 (MQ=255) gctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACaa > 2:822751/1‑81 (MQ=255) ctgGCAGGGGCTAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAAc < 1:822669/81‑1 (MQ=255) ctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACaa < 1:822666/80‑1 (MQ=255) ctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACa < 1:822668/79‑1 (MQ=255) ctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACa < 1:822667/79‑1 (MQ=255) tgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACg > 1:822743/1‑81 (MQ=255) ggCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAAc > 1:822777/1‑79 (MQ=255) ggCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGt > 1:822713/1‑81 (MQ=255) cAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGtgc < 1:822670/81‑1 (MQ=255) aGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGtgct > 1:822757/1‑81 (MQ=255) aGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGtgct < 2:822671/81‑1 (MQ=255) gggCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGtgctgc < 2:822672/81‑1 (MQ=255) gggCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGCCATCGACAACGtgctgc > 2:822746/1‑81 (MQ=255) ccAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGTTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTa > 1:822745/1‑81 (MQ=255) ccAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTa > 2:822792/1‑81 (MQ=255) gTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTAttt > 2:822773/1‑81 (MQ=255) cTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTAttttt > 2:822770/1‑81 (MQ=255) cTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTAttttt > 1:822748/1‑81 (MQ=255) gATCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGtt > 1:822767/1‑81 (MQ=255) aTCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTg < 1:822673/81‑1 (MQ=255) tCAGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGc > 2:822762/1‑81 (MQ=255) aGTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtg > 1:822740/1‑81 (MQ=255) gTTCCTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgt > 2:822744/1‑81 (MQ=255) ttCCTATTTGCTCTCCCTGCGCTGGGTGAACGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgtt < 1:822674/81‑1 (MQ=255) cTATTTGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTc > 2:822768/1‑81 (MQ=255) tGCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGa > 2:822737/1‑81 (MQ=255) gCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGa < 2:822675/80‑1 (MQ=255) gCTCTCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAt > 2:822731/1‑81 (MQ=255) ctctCCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtc < 2:822676/81‑1 (MQ=255) tcCCTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTcc > 1:822756/1‑81 (MQ=255) cTGCGCTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTc < 2:822677/81‑1 (MQ=255) gcgcTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTCTTCCGTTGCTGTTGTCGTGGATCTCCTTCTg > 2:822763/1‑81 (MQ=255) cgcTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCt < 2:822678/79‑1 (MQ=255) cgcTGGGTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTgg > 2:822753/1‑81 (MQ=255) gggTGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGc > 2:822766/1‑81 (MQ=255) gggCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGc > 2:822742/1‑81 (MQ=255) | GATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGC > NC_000913/4076577‑4076731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |