Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,065	A→G	E65G (GAA→GGA)	yaiW →	surface‑exposed outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,065	0	A	G	100.0%	136.7 / NA	39	E65G (GAA→GGA)	yaiW	surface‑exposed outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/19);  total (20/19)

GAGCGTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTAT  >  NC_000913/397988‑398138                                                                              |                                                                          gagcgTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAg                                                                        >  1:90001/1‑81 (MQ=255)     gtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAg                                                                    >  1:89989/1‑81 (MQ=255)            ggCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTgg                                                             >  1:90004/1‑81 (MQ=255)               agaagaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCAc                                                          >  2:90014/1‑81 (MQ=255)                gaagaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCAcc                                                         <  2:89947/81‑1 (MQ=255)                 aagaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCg                                                        <  1:89948/81‑1 (MQ=255)                   gaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGAc                                                      <  1:89949/81‑1 (MQ=255)                   gaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGAc                                                      <  2:89950/81‑1 (MQ=255)                    aTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACg                                                     <  2:89951/81‑1 (MQ=255)                       ccTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCt                                                   <  2:89952/80‑1 (MQ=255)                        cTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTg                                                  >  1:89993/1‑80 (MQ=255)                           cGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGaaa                                              <  1:89953/81‑1 (MQ=255)                               cGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGt                                          <  1:89954/81‑1 (MQ=255)                               cGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGt                                          >  1:90008/1‑81 (MQ=255)                                  gAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTg                                       >  2:90006/1‑81 (MQ=255)                                    aaGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCt                                     <  1:89955/81‑1 (MQ=255)                                     aGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTc                                    <  2:89956/81‑1 (MQ=255)                                      gACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCg                                   <  1:89957/81‑1 (MQ=255)                                        ccGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGt                                 <  1:89958/81‑1 (MQ=255)                                           cgATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCt                              <  1:89959/81‑1 (MQ=255)                                            gATGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTg                             >  2:90003/1‑81 (MQ=255)                                             aTGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCTCCGACGCTGGAAAACGTCTGCTCGGTGCTgg                            >  2:90017/1‑81 (MQ=255)                                              tGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGc                           >  1:90016/1‑81 (MQ=255)                                              tGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGc                           <  2:89960/81‑1 (MQ=255)                                              tGCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGc                           <  2:89961/81‑1 (MQ=255)                                               gCCTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCg                          >  2:89998/1‑81 (MQ=255)                                                ccTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCgg                         <  1:89962/81‑1 (MQ=255)                                                ccTGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCgg                         >  2:89994/1‑81 (MQ=255)                                                  tGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTg                       >  1:90018/1‑81 (MQ=255)                                                  tGGGCGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTg                       >  1:90015/1‑81 (MQ=255)                                                      cGAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGgcgc                   <  1:89963/81‑1 (MQ=255)                                                       gAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGgcg                    >  1:90002/1‑79 (MQ=255)                                                       gAAAGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGgcg                    >  2:89997/1‑79 (MQ=255)                                                         aaGATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCgcagc                <  2:89964/81‑1 (MQ=255)                                                           gATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGcagcag              >  1:617241/1‑81 (MQ=255)                                                           gATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGcagcag              >  1:90011/1‑81 (MQ=255)                                                           gATCTCGCCACCACCTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGcagcag              >  1:90023/1‑81 (MQ=255)                                                                     ccaccTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAAtt    <  1:89965/81‑1 (MQ=255)                                                                        ccTTTGGAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTAt  >  1:90009/1‑80 (MQ=255)                                                                              |                                                                          GAGCGTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTAT  >  NC_000913/397988‑398138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑