Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,387	G→C	G279A (GGT→GCT)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,387	0	G	C	100.0%	138.3 / NA	40	G279A (GGT→GCT)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/22);  total (18/22)

CGGTGGTTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGA  >  NC_000913/4108310‑4108464                                                                              |                                                                              cgGTGGTTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTa                                                                            >  2:831283/1‑81 (MQ=255)  ggtggtTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGTTTACGCTATCGATCTGCTGCTGGCAGCTTAc                                                                           >  2:831286/1‑81 (MQ=255)      gtTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcgtc                                                                        <  1:831234/80‑3 (MQ=255)      gtTATCCGGTGCCTTACGACCGAATTATGGCTTCACGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcggcg                                                                       <  1:831233/81‑1 (MQ=255)       ttCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcggcgg                                                                      <  2:831235/81‑1 (MQ=255)       ttCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcggcgg                                                                      >  1:831300/1‑81 (MQ=255)         ctcCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGt                                                                     <  2:831237/80‑1 (MQ=255)         ctcCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGt                                                                     <  2:831236/80‑1 (MQ=255)         ctcCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTc                                                                    >  2:831298/1‑81 (MQ=255)          tcCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCg                                                                   >  2:831285/1‑81 (MQ=255)            cGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTc                                                                    <  2:831238/78‑1 (MQ=255)            cGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGtt                                                                 <  1:831239/81‑1 (MQ=255)                gCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtgtg                                                              >  2:831297/1‑80 (MQ=255)                     aCGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTa                                                        >  2:831304/1‑81 (MQ=255)                                   gCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACa                                           <  1:831240/80‑1 (MQ=255)                                    cTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGc                                         >  1:831307/1‑81 (MQ=255)                                        ccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTgg                                      <  1:831241/80‑1 (MQ=255)                                        ccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTgg                                      <  2:831242/80‑1 (MQ=255)                                        ccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGt                                     >  1:831303/1‑81 (MQ=255)                                          gTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGtt                                    >  1:831316/1‑80 (MQ=255)                                            aTGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcac                                 >  2:831295/1‑81 (MQ=255)                                               ggCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcaccac                              <  2:831243/81‑1 (MQ=255)                                                gcgcTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACg                             <  1:831244/81‑1 (MQ=255)                                                   cTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAca                          <  2:831245/81‑1 (MQ=255)                                                      aCGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcatc                        <  1:831246/80‑1 (MQ=255)                                                       cGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcatcat                      >  1:831310/1‑81 (MQ=255)                                                       cGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcatcat                      >  2:831311/1‑81 (MQ=255)                                                        gCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACatcatc                     <  2:831248/81‑1 (MQ=255)                                                        gCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACatcatc                     <  1:831247/81‑1 (MQ=255)                                                         cTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACatcatc                     >  2:831305/1‑80 (MQ=255)                                                            tCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGa                   <  2:831249/79‑1 (MQ=255)                                                             cGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGc                >  2:831324/1‑81 (MQ=255)                                                                    ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCt               <  2:831250/75‑1 (MQ=255)                                                                    ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATc            <  1:831252/78‑1 (MQ=255)                                                                    ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCg           <  2:831251/79‑1 (MQ=255)                                                                    ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGa          >  2:831314/1‑80 (MQ=255)                                                                       ctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaaaa       <  1:831253/80‑1 (MQ=255)                                                                        tgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACa     >  1:831317/1‑81 (MQ=255)                                                                          gCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACAt    >  1:831299/1‑80 (MQ=255)                                                                           cAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGa  <  1:831254/81‑1 (MQ=255)                                                                              |                                                                              CGGTGGTTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGA  >  NC_000913/4108310‑4108464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑