Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,142,492	A→T	intergenic (‑271/‑38)	ptsA ← / → frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,492	0	A	T	100.0%	94.0 / NA	27	intergenic (‑271/‑38)	ptsA/frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/16);  total (11/16)

TTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCA  >  NC_000913/4142416‑4142567                                                                             |                                                                            ttACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAatatat                                                                         >  1:840803/1‑81 (MQ=255)    cAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGa                                                                      <  1:840769/81‑1 (MQ=255)       aaGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACAt                                                                   <  2:840770/81‑1 (MQ=255)       aaGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACAt                                                                   >  1:840846/1‑81 (MQ=255)          tCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATggg                                                                >  2:840809/1‑81 (MQ=255)            cAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGc                                                               <  1:840771/80‑1 (MQ=255)            cAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGc                                                               <  2:840772/80‑1 (MQ=255)             aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCtt                                                             >  2:840819/1‑81 (MQ=255)                    ggCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAg                                                      >  1:840813/1‑81 (MQ=255)                     gCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAg                                                      <  1:840773/80‑1 (MQ=255)                          tttATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCgg                                                 >  1:840815/1‑80 (MQ=255)                             aTCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGag                                              >  1:840812/1‑80 (MQ=255)                               ccACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGagcag                                           >  1:840820/1‑81 (MQ=255)                                  cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCtt                                        <  2:840775/81‑1 (MQ=255)                                  cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCtt                                        <  1:840774/81‑1 (MQ=255)                                  cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCtt                                        <  1:840776/81‑1 (MQ=255)                                  cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCt                                         >  1:840808/1‑80 (MQ=255)                                     tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatg                                     <  2:840777/81‑1 (MQ=255)                                     tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTat                                      <  2:840778/80‑1 (MQ=255)                                        gCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaat                                  >  2:840814/1‑81 (MQ=255)                                           ccGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAg                               >  1:840824/1‑81 (MQ=255)                                                  cGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCATGCCCGGGAGCAGCTTATGAATGAGTTGGTg                         >  2:840816/1‑80 (MQ=255)                                                  cGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGc                        <  1:840779/81‑1 (MQ=255)                                                   gATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCa                       <  2:840780/81‑1 (MQ=255)                                                        ccTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATcc                  <  2:840781/81‑1 (MQ=255)                                                               tttCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaa            <  1:840783/80‑1 (MQ=255)                                                               tttCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaa            <  2:840782/80‑1 (MQ=255)                                                                        tCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCa  <  1:840784/81‑1 (MQ=255)                                                                             |                                                                            TTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCA  >  NC_000913/4142416‑4142567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑