Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,894	C→A	A142S (GCA→TCA)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,894	0	C	A	100.0%	162.7 / NA	45	A142S (GCA→TCA)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/21);  total (24/21)

CTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGC  >  NC_000913/4147818‑4147968                                                                             |                                                                           cTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGtt                                                                        >  1:842273/1‑81 (MQ=255)  tGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGttt                                                                       >  2:842275/1‑81 (MQ=255)  tGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGttt                                                                       >  2:842262/1‑81 (MQ=255)   gCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtg                                                                      >  2:842279/1‑81 (MQ=255)   gCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtg                                                                      >  2:842264/1‑81 (MQ=255)     gTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtgct                                                                    >  2:842266/1‑81 (MQ=255)     gTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtgct                                                                    >  1:842293/1‑81 (MQ=255)        aGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTgctgct                                                                 <  1:842210/81‑1 (MQ=255)              tCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCg                                                           <  2:842211/81‑1 (MQ=255)               cTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCgg                                                          >  2:842270/1‑81 (MQ=255)                  gATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCgg                                                          <  1:842212/78‑1 (MQ=255)                     ttGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtgcat                                                    >  1:842269/1‑81 (MQ=255)                      tGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtgcatt                                                   >  2:842283/1‑81 (MQ=255)                      tGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtgcatt                                                   >  1:842298/1‑81 (MQ=255)                            ccAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtgcatttgcatt                                             <  2:842213/81‑1 (MQ=255)                             cAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTgcatttgcattt                                            <  1:842215/81‑1 (MQ=255)                             cAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTgcatttgcattt                                            <  2:842214/81‑1 (MQ=255)                              aaGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTc                                           <  2:842216/81‑1 (MQ=255)                                gCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCAt                                         <  1:842217/81‑1 (MQ=255)                                  aaaTCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTg                                       <  1:842218/81‑1 (MQ=255)                                    aTCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAg                                     >  1:842276/1‑81 (MQ=255)                                       gcgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGt                                  >  2:842281/1‑81 (MQ=255)                                        cgGCAGTGGATAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGtt                                 <  1:842219/81‑1 (MQ=255)                                         ggCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGttt                                <  2:842221/81‑1 (MQ=255)                                         ggCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGttt                                <  1:842220/81‑1 (MQ=255)                                          gCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTg                               <  2:842222/81‑1 (MQ=255)                                             gTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAg                            >  2:842289/1‑81 (MQ=255)                                             gTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAg                            <  1:842223/81‑1 (MQ=255)                                              tGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGc                           <  2:842224/81‑1 (MQ=255)                                                gCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAg                         <  1:842225/81‑1 (MQ=255)                                                 cTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGa                        <  1:842226/81‑1 (MQ=255)                                                   aaGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGaa                       >  1:842286/1‑80 (MQ=255)                                                   aaGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGaa                       >  2:842278/1‑80 (MQ=255)                                                     gCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTGGCATTTAATTGAGCGTTTGTAGCAGaa                       >  1:842285/1‑78 (MQ=255)                                                      cAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGa                   <  2:842227/81‑1 (MQ=255)                                                        ggCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAc                 >  2:842296/1‑81 (MQ=255)                                                          cTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCt               <  2:842228/81‑1 (MQ=255)                                                          cTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCt               <  1:842229/81‑1 (MQ=255)                                                              cgacgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGc           >  2:842291/1‑81 (MQ=255)                                                               gacgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCg          >  2:842280/1‑81 (MQ=255)                                                                acgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGt         <  1:842230/81‑1 (MQ=255)                                                                 cgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTg        >  1:842277/1‑81 (MQ=255)                                                                     aaGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGcc    >  2:842287/1‑81 (MQ=255)                                                                     aaGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGcc    >  2:842301/1‑81 (MQ=255)                                                                       gCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCcgc  >  1:842288/1‑81 (MQ=255)                                                                             |                                                                           CTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGC  >  NC_000913/4147818‑4147968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑