Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,840	A→G	intergenic (+51/‑10)	argB → / → argH	acetylglutamate kinase/argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,840	0	A	G	100.0%	119.1 / NA	35	intergenic (+51/‑10)	argB/argH	acetylglutamate kinase/argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/21);  total (14/21)

GCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACT  >  NC_000913/4156762‑4156913                                                                               |                                                                          gCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGgaa                                                                         >  2:844729/1‑81 (MQ=255)  ccGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAAc                                                                        <  2:844672/81‑1 (MQ=255)      tGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACagag                                                                    >  2:844739/1‑81 (MQ=255)        ggTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGtt                                                                  <  2:844673/81‑1 (MQ=255)        ggTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGt                                                                   >  1:844735/1‑80 (MQ=255)         gTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTa                                                                 <  1:844674/81‑1 (MQ=255)            cgcgGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATgg                                                              <  1:844675/81‑1 (MQ=255)             gcgGATTGTAGCGTAAGTTGTGTTGGCCGGAGGGGCAGCTGTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATggg                                                             <  2:844676/81‑2 (MQ=255)                 aTTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACtt                                                         <  1:844677/81‑1 (MQ=255)                     tAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTggg                                                     >  2:844743/1‑81 (MQ=255)                      aGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTgggg                                                    >  1:844738/1‑81 (MQ=255)                       gCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGgagc                                                   <  2:844678/81‑1 (MQ=255)                             gTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGt                                             <  2:844680/81‑1 (MQ=255)                              ttttGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGtt                                            <  1:844681/81‑1 (MQ=255)                                ttgttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGttt                                           >  1:844750/1‑80 (MQ=255)                                ttgttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGttt                                           <  2:844682/80‑1 (MQ=255)                                ttgttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGttt                                           >  2:844747/1‑80 (MQ=255)                                  gttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAc                                        >  1:844736/1‑81 (MQ=255)                                   ttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAcc                                       <  1:844684/81‑1 (MQ=255)                                   ttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAc                                        <  1:844683/80‑1 (MQ=255)                                      gCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAg                                    >  2:844757/1‑81 (MQ=255)                                       ccGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAgg                                   <  2:844685/81‑1 (MQ=255)                                        cGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGgc                                  <  1:844686/81‑1 (MQ=255)                                         ggAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAgg                                   >  1:844732/1‑79 (MQ=255)                                          gAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGgcag                                >  2:844731/1‑81 (MQ=255)                                             gcgcAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGcagcag                             <  2:844687/81‑1 (MQ=255)                                               gcAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGAt                           <  1:844688/81‑1 (MQ=255)                                                cAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATc                          <  1:844689/81‑1 (MQ=255)                                                cAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATc                          <  1:844690/81‑1 (MQ=255)                                                   cTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAAc                       <  2:844691/81‑1 (MQ=255)                                                         ggCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTc                  >  2:844741/1‑80 (MQ=255)                                                                    tCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCaa       <  2:844692/80‑1 (MQ=255)                                                                    tCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAAc      >  1:844740/1‑81 (MQ=255)                                                                      aaaaTAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGa    >  2:844762/1‑81 (MQ=255)                                                                       aaaTAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGAc   <  1:844693/81‑1 (MQ=255)                                                                        aaTAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACt  >  1:844746/1‑81 (MQ=255)                                                                               |                                                                          GCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACT  >  NC_000913/4156762‑4156913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑