Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,220,231	C→T	R546C (CGC→TGC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,220,231	0	C	T	100.0%	113.1 / NA	32	R546C (CGC→TGC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

CTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAG  >  NC_000913/4220155‑4220304                                                                             |                                                                          cTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGgtgcg                                                                        >  1:861824/1‑80 (MQ=255)    gCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCa                                                                      <  2:861758/79‑1 (MQ=255)           gcCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAAc                                                             <  1:861759/81‑1 (MQ=255)                cccGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCa                                                         >  2:861819/1‑80 (MQ=255)                    cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGt                                                    <  1:861761/81‑1 (MQ=255)                    cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGt                                                    <  2:861760/81‑1 (MQ=255)                    cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGt                                                    <  1:861762/81‑1 (MQ=255)                    cgTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGt                                                    <  1:861763/81‑1 (MQ=255)                       aTTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTg                                                   >  2:861812/1‑79 (MQ=255)                               gCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCAtgt                                         >  2:861818/1‑81 (MQ=255)                               gCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCAtgt                                         >  1:861805/1‑81 (MQ=255)                                cTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCAtgtg                                        >  1:861826/1‑81 (MQ=255)                                cTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCAtgtg                                        >  2:861800/1‑81 (MQ=255)                                   tttGAAGAGATGCATGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGaa                                     <  2:861765/81‑1 (MQ=255)                                   tttGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGaa                                     <  1:861764/81‑1 (MQ=255)                                      gAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGAt                                  >  2:861817/1‑81 (MQ=255)                                        agagATGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGt                                >  1:861796/1‑81 (MQ=255)                                           gaTGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTa                             >  2:861816/1‑81 (MQ=255)                                           gaTGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTa                             <  1:861766/81‑1 (MQ=255)                                            aTGCACGCCGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTAt                            <  2:861767/81‑1 (MQ=255)                                                   ccGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATc                     <  1:861768/81‑1 (MQ=255)                                                   ccGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATc                     <  2:861770/81‑1 (MQ=255)                                                   ccGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATc                     <  2:861769/81‑1 (MQ=255)                                                    cGACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCg                    >  1:861822/1‑81 (MQ=255)                                                     gACCTGTTCCGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCgg                   <  2:861771/81‑1 (MQ=255)                                                             ccGCGCTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGc            <  2:861772/80‑1 (MQ=255)                                                               gcgcTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCa           >  1:861821/1‑79 (MQ=255)                                                               gcgcTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCa           >  2:861829/1‑79 (MQ=255)                                                                 gcTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCa           >  2:861820/1‑77 (MQ=255)                                                                 gcTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCa           >  2:861827/1‑77 (MQ=255)                                                                  cTGATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGAt      >  2:861837/1‑81 (MQ=255)                                                                    gATTACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGAtt     >  1:861775/1‑80 (MQ=255)                                                                      ttACTGGTGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAg  <  1:861773/81‑1 (MQ=255)                                                                             |                                                                          CTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAAAGATTTAG  >  NC_000913/4220155‑4220304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑