| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,221,347 | T→C | I381V (ATT→GTT) | arpA ← | regulator of acetyl CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,221,347 | 0 | T | C | 94.4% | 51.3 / ‑3.9 | 18 | I381V (ATT→GTT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (7/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAATACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTGAATCATCAGTTG > NC_000913/4221269‑4221416 | cTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAacac > 1:861978/1‑81 (MQ=255) gACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACta > 1:861979/1‑81 (MQ=255) aaTTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAg < 1:861949/80‑1 (MQ=255) ttGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCa < 2:861950/81‑1 (MQ=255) ctcAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTc > 1:861982/1‑81 (MQ=255) aaCGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTc < 2:861951/78‑1 (MQ=255) cGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAAGTTAATACTATTATAGGCAGTCAACATTTCTGTTc < 2:861952/81‑1 (MQ=255) gATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCt < 2:861953/81‑1 (MQ=255) tCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGt < 1:861954/81‑1 (MQ=255) ttACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCt < 2:861955/81‑1 (MQ=255) aTTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCAtttt < 2:861956/81‑1 (MQ=255) tataGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTg > 2:861984/1‑81 (MQ=255) gCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGt < 2:861957/81‑1 (MQ=255) cAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGtt > 2:861990/1‑80 (MQ=255) cAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTg < 2:861958/81‑1 (MQ=255) aaaTATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTg > 1:861989/1‑80 (MQ=255) ataAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTGAatca > 2:861988/1‑81 (MQ=255) aaaCCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTGAatcat > 1:861980/1‑80 (MQ=255) gCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTGAATCATCAGTTg < 2:861959/79‑1 (MQ=255) | CTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAATACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAGTTGAATCATCAGTTG > NC_000913/4221269‑4221416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |