| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,222,138 | G→A | T117I (ACT→ATT) | arpA ← | regulator of acetyl CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,222,138 | 0 | G | A | 94.7% | 56.8 / ‑3.8 | 19 | T117I (ACT→ATT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (7/11); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
ATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGT > NC_000913/4222061‑4222211 | aTTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAatgt > 2:862099/1‑81 (MQ=255) ccATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAg < 2:862073/81‑1 (MQ=255) cATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGc < 2:862074/81‑1 (MQ=255) tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGt < 2:862075/80‑1 (MQ=255) cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAtt > 1:862102/1‑81 (MQ=255) ggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTcc < 1:862076/80‑1 (MQ=255) tctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAgg > 1:862107/1‑81 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt < 1:862077/78‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt < 1:862078/78‑1 (MQ=255) ttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCa > 1:862103/1‑80 (MQ=255) ttttAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGc < 2:862079/81‑1 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCaa > 2:862108/1‑81 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCa < 1:862080/80‑1 (MQ=255) aGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATg > 2:862106/1‑81 (MQ=255) aTTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg < 2:862081/81‑1 (MQ=255) ttCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg > 2:862104/1‑80 (MQ=255) gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtg > 2:862109/1‑81 (MQ=255) tATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTc < 1:862082/80‑1 (MQ=255) aTCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCg < 1:862083/80‑1 (MQ=255) tCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgt < 1:862084/80‑1 (MQ=255) | ATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGT > NC_000913/4222061‑4222211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |