Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,400	A→G	F30L (TTT→CTT)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,400	0	A	G	100.0%	26.5 / NA	9	F30L (TTT→CTT)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

CTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGTATTATTAATATTTGCAGAGAAAGAACTACGAGGAATA  >  NC_000913/4222329‑4222476                                                                        |                                                                             ctctGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCAc                                                                      <  2:862107/80‑1 (MQ=255)             ttttAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGgtg                                                         >  2:862119/1‑81 (MQ=255)                tAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGGTGtt                                                       >  1:862121/1‑80 (MQ=255)                  aTAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGGTGTTCAt                                                    >  2:862152/1‑81 (MQ=255)                             gCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCt                                         <  1:862108/81‑1 (MQ=255)                                 gCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGtat                                     <  1:862109/81‑1 (MQ=255)                                     cTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAGCAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGtattat                                  >  1:862124/1‑80 (MQ=255)                                                                 ttATAAAGCAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGTATTATTAATATTTGCAGAGAAAGAACTACGAgg      >  1:862125/1‑80 (MQ=255)                                                                    taAAGCAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGTATTATTAATATTTGCAGAGAAAGAACTACGAGGAATa  <  1:862110/81‑1 (MQ=255)                                                                        |                                                                             CTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCATTTGTCTGGGCTGTATTATTAATATTTGCAGAGAAAGAACTACGAGGAATA  >  NC_000913/4222329‑4222476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑