Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,891	C→T	V115I (GTC→ATC)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,891	0	C	T	100.0%	140.8 / NA	40	V115I (GTC→ATC)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

CACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAC  >  NC_000913/4232818‑4232965                                                                          |                                                                           cACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCa                                                                     <  1:864927/81‑1 (MQ=255)     gtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTg                                                                 >  2:864998/1‑81 (MQ=255)     gtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTg                                                                 >  2:864988/1‑81 (MQ=255)      tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCt                                                                  <  2:864928/79‑1 (MQ=255)      tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTg                                                                 <  2:864929/80‑1 (MQ=255)        ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTc                                                               <  1:864930/80‑1 (MQ=255)        ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCa                                                              <  2:864931/81‑1 (MQ=255)            aaCATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAgc                                                            <  2:864932/79‑1 (MQ=255)             aCATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGcc                                                         <  1:864933/81‑1 (MQ=255)               aTCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCg                                                        >  1:864980/1‑80 (MQ=255)                       cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGtt                                                <  1:864934/80‑1 (MQ=255)                         cgcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGc                                              >  1:864990/1‑80 (MQ=255)                          gcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGc                                              >  1:864992/1‑79 (MQ=255)                           cAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAg                                           >  1:865023/1‑81 (MQ=255)                             ggATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAgcg                                         <  2:864935/81‑1 (MQ=255)                              gATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAgcgc                                        >  1:864994/1‑81 (MQ=255)                              gATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAgcgc                                        >  2:865012/1‑81 (MQ=255)                                tctcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccg                                      >  2:865000/1‑81 (MQ=255)                                 ctcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgc                                     >  1:865015/1‑81 (MQ=255)                                    aacaaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgccgc                                  <  1:864936/81‑1 (MQ=255)                                       aaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTc                               >  2:865005/1‑81 (MQ=255)                                        aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                              >  1:865017/1‑81 (MQ=255)                                        aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                              >  2:865010/1‑81 (MQ=255)                                        aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                              <  2:864937/81‑1 (MQ=255)                                        aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                              <  2:864938/81‑1 (MQ=255)                                          cagcagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGc                            >  2:864997/1‑81 (MQ=255)                                           agcagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGcc                           <  2:864939/81‑1 (MQ=255)                                             cagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGTCAg                         <  1:864940/81‑1 (MQ=255)                                             cagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAg                         <  1:864941/81‑1 (MQ=255)                                              agGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGa                        <  1:864942/81‑1 (MQ=255)                                                  tCGACATCAGCCCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCCTCTGCCAGAACAg                    >  2:865022/1‑81 (MQ=255)                                                    gACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGt                   <  2:864943/80‑1 (MQ=255)                                                      cATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAAt                <  2:864945/81‑1 (MQ=255)                                                      cATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAAt                <  2:864944/81‑1 (MQ=255)                                                      cATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAAt                >  2:865004/1‑81 (MQ=255)                                                          aGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTtc            <  1:864946/81‑1 (MQ=255)                                                             tCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTtctc          >  1:864999/1‑80 (MQ=255)                                                                ccGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCcagc      <  1:864947/81‑1 (MQ=255)                                                                  gTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCcag       >  1:865016/1‑78 (MQ=255)                                                                    ggCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc  >  1:865019/1‑81 (MQ=255)                                                                          |                                                                           CACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAC  >  NC_000913/4232818‑4232965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑