Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,234,701	T→C	V315A (GTA→GCA)	pgi →	glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,701	0	T	C	100.0%	136.7 / NA	40	V315A (GTA→GCA)	pgi	glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

ACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAG  >  NC_000913/4234623‑4234777                                                                               |                                                                             aCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCAc                                                                            >  2:865470/1‑81 (MQ=255) aCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCAc                                                                            <  2:865405/81‑1 (MQ=255)      gTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCActgctg                                                                       <  1:865406/81‑1 (MQ=255)       ttGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTgg                                                                      >  2:865452/1‑81 (MQ=255)         gAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGgc                                                                     <  2:865408/80‑1 (MQ=255)         gAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGgc                                                                     <  2:865407/80‑1 (MQ=255)                   ccGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGc                                                           <  2:865409/80‑1 (MQ=255)                    cGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCAt                                                         >  2:865453/1‑81 (MQ=255)                     ggCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATc                                                        <  2:865410/81‑1 (MQ=255)                      gcgcACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCt                                                       <  1:865411/81‑1 (MQ=255)                      gcgcACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCt                                                       <  1:865412/81‑1 (MQ=255)                          acacGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTa                                                   >  1:865458/1‑81 (MQ=255)                            acGCGATGGACAAGCATTTCTCCACCACGCCTGTCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTac                                                  >  2:865478/1‑80 (MQ=255)                            acGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGTCATCTGGTac                                                  >  1:865479/1‑80 (MQ=255)                            acGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTac                                                  >  1:865455/1‑80 (MQ=255)                            acGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTac                                                  >  1:865474/1‑80 (MQ=255)                             cgcgATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTacaa                                                <  2:865413/81‑1 (MQ=255)                              gcgATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTacaa                                                >  1:865460/1‑80 (MQ=255)                                gATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTAcaacaa                                             >  2:865464/1‑81 (MQ=255)                                  tGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAAtt                                           >  1:865454/1‑81 (MQ=255)                                   ggACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAAttt                                          >  1:865462/1‑81 (MQ=255)                                    gACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTc                                         >  2:865477/1‑81 (MQ=255)                                     aCAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCt                                        >  2:865463/1‑81 (MQ=255)                                     aCAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCt                                        <  2:865415/81‑1 (MQ=255)                                     aCAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCt                                        <  2:865414/81‑1 (MQ=255)                                     aCAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCt                                        >  1:865459/1‑81 (MQ=255)                                       aaGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTAGCATCTGGTACAACAATTTCttt                                      <  1:865416/81‑1 (MQ=255)                                            tttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGc                                 <  1:865418/81‑1 (MQ=255)                                            tttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGc                                 <  1:865417/81‑1 (MQ=255)                                                  caccacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGaaa                            <  1:865420/80‑1 (MQ=255)                                                  caccacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGaaa                            <  1:865421/80‑1 (MQ=255)                                                  caccacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGaa                             <  2:865419/79‑1 (MQ=255)                                                   accacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACt                          >  2:865467/1‑81 (MQ=255)                                                   accacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACt                          >  1:865456/1‑81 (MQ=255)                                                   accacGCCTGCCGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACt                          <  1:865422/81‑1 (MQ=255)                                                             ccGAGAAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGAt                  <  2:865423/79‑1 (MQ=255)                                                                 gaAAAACCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGcc            <  2:865424/81‑1 (MQ=255)                                                                   aaaaCCTGCCTGCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCg           >  1:865473/1‑80 (MQ=255)                                                                       cctgcctgCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGa      >  1:865476/1‑81 (MQ=255)                                                                           cctgCACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAg  >  2:865485/1‑81 (MQ=255)                                                                               |                                                                             ACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAG  >  NC_000913/4234623‑4234777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑