Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,252,298	C→T	intergenic (+15/‑208)	yjbI → / → ubiC	pentapeptide repeats‑containing protein YjbI/chorismate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,252,298	0	C	T	100.0%	96.0 / NA	27	intergenic (+15/‑208)	yjbI/ubiC	pentapeptide repeats‑containing protein YjbI/chorismate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/10);  total (17/10)

TTTCCCCATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGG  >  NC_000913/4252226‑4252372                                                                         |                                                                           tttCCCCATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTcc                                                                    >  1:869786/1‑81 (MQ=255)  ttCCCCATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTc                                                                     <  1:869764/79‑1 (MQ=255)  ttCCCCATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCt                                                                   >  2:869802/1‑81 (MQ=255)     cccATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTgg                                                                 <  1:869765/80‑1 (MQ=255)       cATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCAt                                                              >  1:869805/1‑81 (MQ=255)         tttttACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATcc                                                            <  1:869766/81‑1 (MQ=255)              aCTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGAc                                                       >  2:869797/1‑81 (MQ=255)                 gCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGt                                                    >  1:869793/1‑81 (MQ=255)                    aGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGAt                                                 <  2:869767/81‑1 (MQ=255)                    aGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGAt                                                 >  2:869807/1‑81 (MQ=255)                        ggCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGccc                                             >  2:869795/1‑81 (MQ=255)                         gCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGcccc                                            >  2:869827/1‑81 (MQ=255)                                cATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGtt                                     >  2:869810/1‑81 (MQ=255)                                      ataATGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTc                               >  2:869816/1‑81 (MQ=255)                                        aaTGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCTTGGACGGTGATGCCCCTACGGTTGCCCTCGc                             >  2:869798/1‑81 (MQ=255)                                         aTGAATAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGcc                            <  1:869768/81‑1 (MQ=255)                                             aTAAGGGTGTTTAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCa                        >  1:869796/1‑81 (MQ=255)                                                      tttAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATc                <  1:869769/80‑1 (MQ=255)                                                        tAAGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCgg              >  1:869822/1‑80 (MQ=255)                                                          aGTAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTaa           <  2:869770/81‑1 (MQ=255)                                                            tAAAGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTagag         >  2:869806/1‑81 (MQ=255)                                                             aaaGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGc        >  2:869814/1‑81 (MQ=255)                                                              aaGGAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCg       >  1:869831/1‑81 (MQ=255)                                                                ggAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTa     <  1:869771/81‑1 (MQ=255)                                                                ggAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTa     <  2:869772/81‑1 (MQ=255)                                                                 gAAAACATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTaa    <  2:869773/81‑1 (MQ=255)                                                                   aaaCATTACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAgg  >  1:869819/1‑81 (MQ=255)                                                                         |                                                                           TTTCCCCATTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGG  >  NC_000913/4252226‑4252372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑