Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,489	C→T	P326P (CCG→CCA)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,489	0	C	T	100.0%	175.5 / NA	48	P326P (CCG→CCA)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/20);  total (28/20)

ATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACAT  >  NC_000913/4255412‑4255562                                                                              |                                                                          aTAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCggtggc                                                                        <  2:870694/81‑1 (MQ=255) aTAAAGAACGCCCCCAGACGGCGGAAAATCGACCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCggtggc                                                                        <  2:870695/81‑1 (MQ=255)  tAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCa                                                                       >  1:870823/1‑81 (MQ=255)   aaaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCAc                                                                      >  2:870784/1‑81 (MQ=255)   aaaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCAc                                                                      >  2:870696/1‑81 (MQ=255)   aaaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCAc                                                                      >  1:870752/1‑81 (MQ=255)   aaaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATACGGTGGCAc                                                                      >  2:870772/1‑81 (MQ=255)    aaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCAc                                                                      >  1:870789/1‑80 (MQ=255)     aGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCAc                                                                      >  1:870786/1‑79 (MQ=255)       aaCGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGc                                                                  >  2:870758/1‑81 (MQ=255)         cGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGccc                                                                <  2:870697/81‑1 (MQ=255)          gCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGcccc                                                               <  2:870698/81‑1 (MQ=255)            ccccAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGCCGGCGATATGCGGTGGCACCAGCCCCtt                                                             <  1:870699/81‑2 (MQ=255)             cccAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTgg                                                            <  1:870700/81‑1 (MQ=255)              ccAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGt                                                           <  2:870701/81‑1 (MQ=255)                 gACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGa                                                         >  2:870761/1‑80 (MQ=255)                   cggcggAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATAc                                                      <  2:870702/81‑1 (MQ=255)                    ggcggAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATa                                                       >  1:870782/1‑79 (MQ=255)                    ggcggAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACa                                                     <  1:870703/81‑1 (MQ=255)                     gcggAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAg                                                    >  2:870733/1‑81 (MQ=255)                        gAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCAc                                                 <  2:870704/81‑1 (MQ=255)                         aaaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACg                                                <  1:870705/81‑1 (MQ=255)                               ggCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGaa                                          <  2:870706/81‑1 (MQ=255)                                 cccGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAag                                        >  2:870792/1‑81 (MQ=255)                                  ccGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAag                                        >  1:870795/1‑80 (MQ=255)                                  ccGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAag                                        >  1:870794/1‑80 (MQ=255)                                   cGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAag                                        >  1:870769/1‑79 (MQ=255)                                   cGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAag                                        >  1:870790/1‑79 (MQ=255)                                     gCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAagcag                                     >  1:870806/1‑80 (MQ=255)                                      cAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAagcag                                     >  2:870768/1‑79 (MQ=255)                                       aGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGcagcag                                  <  1:870707/81‑1 (MQ=255)                                        ggCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAgg                                 <  2:870708/81‑1 (MQ=255)                                             gAAATTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTc                            >  1:870785/1‑81 (MQ=255)                                              aaaTTCAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTcc                           <  2:870709/81‑1 (MQ=255)                                                   cAGGTTGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgt                       >  2:870775/1‑80 (MQ=255)                                                       ttGATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTg                  >  2:870783/1‑81 (MQ=255)                                                         gATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGc                 <  2:870710/80‑1 (MQ=255)                                                         gATCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                >  2:870777/1‑81 (MQ=255)                                                           tCCCGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGt              <  1:870711/81‑1 (MQ=255)                                                            cccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTg             >  2:870757/1‑81 (MQ=255)                                                            cccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTg             >  2:870801/1‑81 (MQ=255)                                                             ccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTgg            <  2:870715/81‑1 (MQ=255)                                                             ccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTgg            <  1:870712/81‑1 (MQ=255)                                                             ccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTgg            <  1:870713/81‑1 (MQ=255)                                                             ccGGCGGCGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTg             <  2:870714/80‑1 (MQ=255)                                                               ggcggcGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc           >  1:870778/1‑80 (MQ=255)                                                               ggcggcGATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc           >  1:870780/1‑80 (MQ=255)                                                                  ggcgATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAgg       >  2:870804/1‑81 (MQ=255)                                                                     gATATGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGca     >  1:870807/1‑80 (MQ=255)                                                                       tatGCGGTGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACat  <  1:870716/81‑1 (MQ=255)                                                                              |                                                                          ATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACAT  >  NC_000913/4255412‑4255562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑