Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,259,861	A→G	L48P (CTT→CCT)	zur ←	DNA‑binding transcriptional repressor Zur

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,259,861	0	A	G	100.0%	120.7 / NA	35	L48P (CTT→CCT)	zur	DNA‑binding transcriptional repressor Zur
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

CTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCA  >  NC_000913/4259784‑4259933                                                                              |                                                                         cTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCa                                                                       <  2:871863/81‑1 (MQ=255)     cTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGAt                                                                    <  1:871864/80‑1 (MQ=255)     cTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGAt                                                                    >  2:871906/1‑80 (MQ=255)     cTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGAt                                                                    >  1:871898/1‑80 (MQ=255)        aaGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCa                                                                  <  1:871865/79‑1 (MQ=255)             gAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAgcgc                                                           >  2:871915/1‑81 (MQ=255)                aaTCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGa                                                        >  2:871904/1‑81 (MQ=255)                 aTCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGa                                                        >  2:871933/1‑80 (MQ=255)                 aTCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGAt                                                       <  1:871866/81‑1 (MQ=255)                 aTCCAGCGCGCGATAAACCGTTGGCGGCTTGACTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGAt                                                       <  2:871867/81‑1 (MQ=255)                    cAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAgc                                                    >  1:871918/1‑81 (MQ=255)                       cgcgcgATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGcc                                                 <  1:871868/81‑1 (MQ=255)                          gcgATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATc                                              >  1:871910/1‑81 (MQ=255)                           cgATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATcc                                             >  1:871920/1‑81 (MQ=255)                                 aCCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGa                                       <  2:871869/81‑1 (MQ=255)                                  ccGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGAc                                      >  2:871928/1‑81 (MQ=255)                                          ggcttggcttGCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAgg                              >  2:871924/1‑81 (MQ=255)                                           gcttggcttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAgc                              >  2:871870/1‑79 (MQ=255)                                           gcttggcttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGgc                             >  2:871917/1‑81 (MQ=255)                                            cttggcttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGgcg                            >  2:871932/1‑81 (MQ=255)                                               ggcttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGgcgc                           <  1:871871/79‑1 (MQ=255)                                               ggcttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCaa                         >  2:871925/1‑81 (MQ=255)                                                 cttgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAaca                       <  2:871872/81‑1 (MQ=255)                                                   tgCGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACt                     >  1:871921/1‑81 (MQ=255)                                                     cGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTc                   <  1:871873/81‑1 (MQ=255)                                                     cGGTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGACATCCTGCAGACTCATCAGGCGCAACACtt                    <  1:871874/80‑1 (MQ=255)                                                      ggTTCAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTc                   >  1:871916/1‑80 (MQ=255)                                                          cAGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGgc              >  2:871929/1‑81 (MQ=255)                                                           aGCTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGgcg             >  1:871927/1‑81 (MQ=255)                                                           aGCTTCGCGCAGTAAATCAGGCAGATCATAAGCACTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGgc              <  2:871875/80‑1 (MQ=255)                                                             cTTCGCGCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCt           >  2:871919/1‑81 (MQ=255)                                                                cgcgCAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCtgtg        >  1:871930/1‑81 (MQ=255)                                                                 gcgcAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTgg       <  1:871876/81‑1 (MQ=255)                                                                    cAGTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGt    >  2:871923/1‑81 (MQ=255)                                                                      gTAAATCAGGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCa  <  2:871877/81‑1 (MQ=255)                                                                              |                                                                         CTTGCTCAAGCAGAAAATCCAGCGCGCGATAAACCGTTGGCGGCTTGGCTTGCGGTTCAGCTTCGCGCAGTAAATCAAGCAGATCATAAGCGCTGATAGCGCCATCCTGCAGACTCATCAGGCGCAACACTTCCAGGCGCTGTGGGGTCA  >  NC_000913/4259784‑4259933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑