Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,273,365	C→A	L169L (CTG→CTT)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,273,365	0	C	A	100.0%	175.3 / NA	48	L169L (CTG→CTT)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/22);  total (26/22)

GTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGCTTGCCTTC  >  NC_000913/4273292‑4273442                                                                          |                                                                              gTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTtct                                                                        <  1:875035/81‑1 (MQ=255)  ttCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTtctc                                                                       >  2:875095/1‑81 (MQ=255)  ttCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTtctc                                                                       >  1:875090/1‑81 (MQ=255)   tCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTtctc                                                                       >  2:875081/1‑80 (MQ=255)     cAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCa                                                                     <  1:875036/80‑1 (MQ=255)     cAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAg                                                                    >  2:875101/1‑81 (MQ=255)       gTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCg                                                                  <  1:875037/81‑1 (MQ=255)         tttGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGtt                                                                >  1:875088/1‑81 (MQ=255)          ttGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGtt                                                                <  2:875038/80‑1 (MQ=255)             gCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTg                                                             <  1:875039/80‑1 (MQ=255)              cGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGgt                                                           <  2:875040/81‑1 (MQ=255)                gATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGgtgt                                                         >  2:875099/1‑81 (MQ=255)                 aTCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGgtgtg                                                        >  1:875083/1‑81 (MQ=255)                  tCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGgtgtgt                                                       <  1:875041/81‑1 (MQ=255)                    ggAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGtt                                                      <  1:875043/80‑1 (MQ=255)                    ggAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTc                                                     >  2:875149/1‑81 (MQ=255)                    ggAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTc                                                     <  1:875042/81‑1 (MQ=255)                    ggAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTc                                                     <  1:875044/81‑1 (MQ=255)                     gAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCg                                                    <  2:875045/81‑1 (MQ=255)                        aGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCt                                                 >  1:875110/1‑81 (MQ=255)                         gATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCtt                                                <  1:875046/81‑1 (MQ=255)                         gATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCtt                                                <  1:875047/81‑1 (MQ=255)                         gATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCtt                                                <  1:875048/81‑1 (MQ=255)                             gCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTgc                                             <  2:875049/80‑1 (MQ=255)                              cAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTgcgc                                           <  2:875050/81‑1 (MQ=255)                                 aCTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGctct                                        <  2:875051/81‑1 (MQ=255)                                    tCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCttt                                      >  2:875092/1‑80 (MQ=255)                                     cGCCATCAATACGAGCACGGATGTAGCTCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTaa                                    >  1:875109/1‑81 (MQ=255)                                     cGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTaa                                    >  1:875100/1‑81 (MQ=255)                                     cGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTa                                     <  1:875052/80‑1 (MQ=255)                                     cGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTa                                     <  2:875053/80‑1 (MQ=255)                                      gCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAAt                                   >  1:875114/1‑81 (MQ=255)                                       ccATCAATACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAAt                                   >  1:875102/1‑80 (MQ=255)                                            aaTACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTgg                             >  1:875103/1‑81 (MQ=255)                                            aaTACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTgg                             >  1:875089/1‑81 (MQ=255)                                            aaTACGAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTgg                             >  1:875097/1‑81 (MQ=255)                                                 gAGCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGgcgc                          >  1:875096/1‑79 (MQ=255)                                                   gCACGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAg                       >  2:875132/1‑80 (MQ=255)                                                      cGGATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCTTTTGCGCTCTTTAATGATTGGCGCGAGTAg                    >  2:875122/1‑80 (MQ=255)                                                       ggATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCa                  >  2:875133/1‑81 (MQ=255)                                                       ggATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCa                  >  2:875107/1‑81 (MQ=255)                                                        gATGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCAt                 >  1:875094/1‑81 (MQ=255)                                                         aTGTAGCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATc                <  1:875054/81‑1 (MQ=255)                                                              gCCCTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACg           <  2:875055/81‑1 (MQ=255)                                                               cccTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGc          >  1:875108/1‑81 (MQ=255)                                                                 cTGGCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGCtt        <  2:875056/81‑1 (MQ=255)                                                                   ggCTTGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGCTTGc      >  2:875098/1‑81 (MQ=255)                                                                       tGCAAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGCTTGCCTTc  >  2:875113/1‑81 (MQ=255)                                                                          |                                                                              GTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGTTCTCCAGCGTTTTGGTGTGTTCGCCTTTGCGCTCTTTAATGATTGGCGCGAGTAGCATCAGACGCTTGCCTTC  >  NC_000913/4273292‑4273442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑