Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,975	T→C	I310V (ATT→GTT)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,975	0	T	C	100.0%	158.1 / NA	46	I310V (ATT→GTT)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

ATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAG  >  NC_000913/4283898‑4284051                                                                              |                                                                             aTGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAAcata                                                                           <  1:877468/81‑1 (MQ=255)    gCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGa                                                                         <  1:877469/80‑1 (MQ=255)       cgccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTa                                                                     <  1:877470/81‑1 (MQ=255)        gccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAg                                                                    >  1:877531/1‑81 (MQ=255)         ccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGc                                                                   <  2:877471/81‑1 (MQ=255)           gcgTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGcc                                                                  >  2:877522/1‑80 (MQ=255)             gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCAt                                                               <  1:877472/81‑1 (MQ=255)              tCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATa                                                              <  2:877473/81‑1 (MQ=255)               cTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATa                                                              >  1:877524/1‑80 (MQ=255)                    tatTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAAccc                                                         <  1:877474/80‑1 (MQ=255)                      ttCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCgg                                                       <  1:877475/80‑1 (MQ=255)                        ccGGATTCGCACCAGCCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTg                                                     <  2:877476/80‑1 (MQ=255)                         cGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGc                                                   <  2:877477/81‑1 (MQ=255)                               cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGaa                                             >  2:877547/1‑81 (MQ=255)                               cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGaa                                             >  2:877530/1‑81 (MQ=255)                                gCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGaa                                             <  2:877478/80‑1 (MQ=255)                                gCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAac                                            <  1:877479/81‑1 (MQ=255)                                  accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAacac                                          >  1:877535/1‑81 (MQ=255)                                  accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAacac                                          <  2:877480/81‑1 (MQ=255)                                     aaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGct                                       >  2:877513/1‑81 (MQ=255)                                     aaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGct                                       <  2:877481/81‑1 (MQ=255)                                        caGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGctct                                     >  2:877534/1‑80 (MQ=255)                                           cATGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACg                                 >  1:877545/1‑81 (MQ=255)                                            aTGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGt                                <  2:877482/81‑1 (MQ=255)                                             tGATCGCGCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTg                               <  2:877483/81‑1 (MQ=255)                                                 cgcgCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTc                           >  2:877566/1‑81 (MQ=255)                                                 cgcgCCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTc                           >  2:877523/1‑81 (MQ=255)                                                  gcgcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcg                          >  1:877555/1‑81 (MQ=255)                                                  gcgcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTc                           <  1:877485/80‑1 (MQ=255)                                                  gcgcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTc                           <  1:877484/80‑1 (MQ=255)                                                    gcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                        >  2:877540/1‑81 (MQ=255)                                                    gcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                        >  1:877525/1‑81 (MQ=255)                                                    gcCGAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                        >  2:877529/1‑81 (MQ=255)                                                       gAAGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCggg                      >  2:877550/1‑80 (MQ=255)                                                        aaGCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAATCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCa                    >  2:877537/1‑81 (MQ=255)                                                          gCCGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATc                  <  2:877486/81‑1 (MQ=255)                                                            cGATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGc                >  2:877542/1‑81 (MQ=255)                                                             gATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt               <  2:877487/81‑1 (MQ=255)                                                             gATAATAAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt               <  2:877488/81‑1 (MQ=255)                                                              ataataAAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTg              <  1:877489/81‑1 (MQ=255)                                                                  taaAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTg          <  2:877491/81‑1 (MQ=255)                                                                  taaAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTg          <  2:877490/81‑1 (MQ=255)                                                                  taaAGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTg          >  2:877544/1‑81 (MQ=255)                                                                   aaaGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTg          >  2:877536/1‑80 (MQ=255)                                                                    aaGGTCAGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGt         <  1:877492/80‑1 (MQ=255)                                                                          aGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaag  >  1:877541/1‑81 (MQ=255)                                                                          aGAACATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaag  >  1:877560/1‑81 (MQ=255)                                                                              |                                                                             ATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAG  >  NC_000913/4283898‑4284051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑