Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,936	A→G	E140G (GAA→GGA)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,936	0	A	G	100.0%	119.3 / NA	36	E140G (GAA→GGA)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/22);  total (14/22)

TCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCA  >  NC_000913/422861‑423013                                                                            |                                                                              tCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCt                                                                           <  2:95029/80‑1 (MQ=255) tCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCt                                                                           <  2:95028/80‑1 (MQ=255)   ggCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGaa                                                                        >  1:95079/1‑81 (MQ=255)         caATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGAt                                                                  <  2:95030/81‑1 (MQ=255)              ggCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGt                                                             >  2:95075/1‑81 (MQ=255)                  gCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTAt                                                         >  2:95087/1‑81 (MQ=255)                   cGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTAt                                                         <  1:95031/80‑1 (MQ=255)                      aTCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTAcc                                                     <  2:95032/81‑1 (MQ=255)                           aTTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACcatcat                                                <  2:95033/81‑1 (MQ=255)                             tttttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATg                                               >  2:95090/1‑80 (MQ=255)                              ttttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATg                                               >  2:95071/1‑79 (MQ=255)                              ttttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGc                                              <  2:95034/80‑1 (MQ=255)                               tttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCa                                             <  1:95036/80‑1 (MQ=255)                               tttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCa                                             <  2:95035/80‑1 (MQ=255)                                ttATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAg                                            <  2:95037/80‑1 (MQ=255)                                  aTCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGcc                                          <  1:95038/80‑1 (MQ=255)                                         cTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACaa                                    >  1:95076/1‑79 (MQ=255)                                         cTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACaa                                    >  2:95070/1‑79 (MQ=255)                                           tCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACaa                                    >  2:95072/1‑77 (MQ=255)                                            cccTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACaa                                    <  2:95041/76‑1 (MQ=255)                                            cccTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAagag                                 <  2:95040/79‑1 (MQ=255)                                            cccTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAag                                   <  2:95039/77‑1 (MQ=255)                                             ccTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGat                               <  1:95043/80‑1 (MQ=255)                                             ccTGATCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGat                               <  1:95042/80‑1 (MQ=255)                                                 aTCGTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGatcat                            <  1:95044/79‑1 (MQ=255)                                                    gTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGc                       >  2:95077/1‑81 (MQ=255)                                                    gTTTTGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGc                       >  2:95083/1‑81 (MQ=255)                                                        tGCGCGTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGa                   >  1:95084/1‑81 (MQ=255)                                                           gcgTAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTg                >  1:95091/1‑81 (MQ=255)                                                              tAGTCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGa             <  2:95045/81‑1 (MQ=255)                                                                 tCTTCCTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGa          <  1:95046/81‑1 (MQ=255)                                                                     ccTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAAc        <  1:95047/79‑1 (MQ=255)                                                                     ccTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCg      >  1:95085/1‑81 (MQ=255)                                                                      cTCGTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCg      <  2:95048/80‑1 (MQ=255)                                                                         gTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTc   <  2:95049/80‑1 (MQ=255)                                                                         gTGGAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTCCGTGACTGGGATGAACCGGTCa  >  1:95078/1‑81 (MQ=255)                                                                            |                                                                              TCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCA  >  NC_000913/422861‑423013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑