Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,871	T→C	Q499R (CAG→CGG)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,871	0	T	C	100.0%	97.0 / NA	29	Q499R (CAG→CGG)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/11);  total (18/11)

AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTT  >  NC_000913/4297799‑4297941                                                                         |                                                                       aGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttg                                                                <  1:881171/81‑1 (MQ=255)  gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgt                                                               >  2:881213/1‑81 (MQ=255)     cGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgttg                                                             >  1:881217/1‑80 (MQ=255)      gCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgttgt                                                            <  1:881172/80‑1 (MQ=255)          aTTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTg                                                        >  2:881201/1‑80 (MQ=255)          aTTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGc                                                       <  2:881173/81‑1 (MQ=255)            tttCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCAt                                                     >  2:881227/1‑81 (MQ=255)            tttCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCAt                                                     >  2:881205/1‑81 (MQ=255)               ctcGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCg                                                   >  2:881202/1‑80 (MQ=255)                 cGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGa                                                 >  1:881203/1‑80 (MQ=255)                  gTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGAt                                                >  2:881226/1‑80 (MQ=255)                    aaGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAAc                                             >  1:881237/1‑81 (MQ=255)                      gTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAAccc                                           <  1:881174/81‑1 (MQ=255)                        cGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCa                                          >  1:881225/1‑80 (MQ=255)                            ccGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                                     <  2:881175/81‑1 (MQ=255)                                 gAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                <  1:881177/81‑1 (MQ=255)                                 gAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                <  1:881176/81‑1 (MQ=255)                                  aaaTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                >  2:881222/1‑80 (MQ=255)                                  aaaTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCg                               <  2:881178/81‑1 (MQ=255)                                        gggCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa                         >  2:881210/1‑81 (MQ=255)                                         ggCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAc                        >  1:881234/1‑81 (MQ=255)                                          gCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTTTATTGCATCGGATAACCCATACTGGTGGCGATTTCACt                       >  2:881216/1‑81 (MQ=255)                                            acaGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtga                     <  2:881179/81‑1 (MQ=255)                                             caGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATAGGATAACCCATACGGGTGGCGATTTCACtgat                    <  1:881180/81‑1 (MQ=255)                                                  gACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAttt               >  1:881231/1‑81 (MQ=255)                                                             tCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtt    <  2:881181/81‑1 (MQ=255)                                                                cAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtttt  >  2:881235/1‑80 (MQ=255)                                                                   aTCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtttt  >  1:881219/1‑77 (MQ=255)                                                                    tctcCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtttt  >  2:881218/1‑76 (MQ=255)                                                                         |                                                                       AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTT  >  NC_000913/4297799‑4297941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑