Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,401	T→C	N374S (AAC→AGC)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,401	0	T	C	100.0%	135.5 / NA	40	N374S (AAC→AGC)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/16);  total (24/16)

GTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACCAGGC  >  NC_000913/4339324‑4339477                                                                              |                                                                             gTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCtgt                                                                           >  2:892270/1‑81 (MQ=255)    cATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGa                                                                         <  1:892217/80‑1 (MQ=255)     aTGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATc                                                                       <  2:892218/81‑1 (MQ=255)     aTGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATc                                                                       <  1:892220/81‑1 (MQ=255)     aTGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATc                                                                       <  1:892219/81‑1 (MQ=255)        aaTATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGc                                                                     >  2:892257/1‑80 (MQ=255)            taAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAgg                                                                 <  2:892221/80‑1 (MQ=255)              agaagCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTc                                                              >  1:892274/1‑81 (MQ=255)               gaagCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTcg                                                             >  2:892285/1‑81 (MQ=255)               gaagCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTcg                                                             <  2:892222/81‑1 (MQ=255)                   ccTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTcgccgc                                                         <  2:892223/81‑1 (MQ=255)                    cTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCcgcg                                                        >  2:892282/1‑81 (MQ=255)                      gtgACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCa                                                      >  2:892267/1‑81 (MQ=255)                       tgACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCgcgc                                                       <  1:892224/79‑1 (MQ=255)                        gACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATg                                                    >  1:892275/1‑81 (MQ=255)                          cAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATgg                                                   >  2:892283/1‑80 (MQ=255)                          cAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGc                                                  <  1:892225/81‑1 (MQ=255)                              gcATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAg                                              >  1:892296/1‑81 (MQ=255)                              gcATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAg                                              >  2:892263/1‑81 (MQ=255)                                aTTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGt                                             >  1:892317/1‑80 (MQ=255)                                  tCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGAt                                          >  2:892281/1‑81 (MQ=255)                                   cagcagTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATc                                         >  1:892301/1‑81 (MQ=255)                                     gcagTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                                       >  1:892256/1‑81 (MQ=255)                                        gTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCa                                     >  1:892295/1‑80 (MQ=255)                                        gTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCa                                     >  1:892265/1‑80 (MQ=255)                                           tgtgGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCa                                     >  2:892280/1‑77 (MQ=255)                                                   gAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTg                         <  1:892226/81‑1 (MQ=255)                                                      tgggtggCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGaaa                      >  2:892288/1‑81 (MQ=255)                                                            gCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCa                >  1:892290/1‑81 (MQ=255)                                                             cGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCAt               <  1:892227/81‑1 (MQ=255)                                                              gAAAACGGTAGGACCGCTGTGATCGCTAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCa                >  1:892276/1‑79 (MQ=255)                                                               aaaaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAg             >  1:892279/1‑81 (MQ=255)                                                                aaaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGc            >  1:892304/1‑81 (MQ=255)                                                                aaaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGc            >  2:892278/1‑81 (MQ=255)                                                                  aCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCg          <  1:892228/81‑1 (MQ=255)                                                                    ggTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTa        >  1:892286/1‑81 (MQ=255)                                                                    ggTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTa        <  2:892229/81‑1 (MQ=255)                                                                    ggTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTa        <  1:892230/81‑1 (MQ=255)                                                                         gACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACCAgg   <  1:892231/81‑1 (MQ=255)                                                                          aCCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACCAGGc  <  2:892232/81‑1 (MQ=255)                                                                              |                                                                             GTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACCAGGC  >  NC_000913/4339324‑4339477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑