Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,746	C→T	L333L (CTG→CTA)	dcuB ←	anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,746	0	C	T	100.0%	134.2 / NA	38	L333L (CTG→CTA)	dcuB	anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/25);  total (13/25)

GCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAT  >  NC_000913/4347669‑4347823                                                                              |                                                                              gCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGt                                                                            <  1:894361/81‑1 (MQ=255)   cTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTAc                                                                          <  1:894362/81‑1 (MQ=255)       gagTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGccc                                                                      <  1:894363/81‑1 (MQ=255)               aaaCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTc                                                              <  1:894364/81‑1 (MQ=255)                 aCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAg                                                            <  2:894365/81‑1 (MQ=255)                     ggAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGAc                                                          <  1:894366/79‑1 (MQ=255)                       aaaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACatat                                                      <  2:894367/81‑1 (MQ=255)                        aaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATg                                                     >  2:894428/1‑81 (MQ=255)                        aaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATg                                                     <  1:894368/81‑1 (MQ=255)                              cagcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCAcc                                               <  1:894369/81‑1 (MQ=255)                              cagcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCAcc                                               <  1:894370/81‑1 (MQ=255)                               agcagAACAATGGCATAGGCCCACGGATACTCTTTGACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCg                                              <  1:894371/81‑1 (MQ=255)                                gcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCg                                              >  1:894426/1‑80 (MQ=255)                                gcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCg                                              >  2:894408/1‑80 (MQ=255)                                gcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCg                                              >  1:894422/1‑80 (MQ=255)                                gcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGa                                             <  1:894372/81‑1 (MQ=255)                                 cagAACAATGGCATAGGACAACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGaa                                            <  1:894373/81‑1 (MQ=255)                                  agAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAAc                                           <  2:894374/81‑1 (MQ=255)                                    aaCAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACAt                                         >  1:894403/1‑81 (MQ=255)                                        aTGGCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGtt                                     >  2:894407/1‑81 (MQ=255)                                           gCATAGGCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCt                                  <  1:894375/81‑1 (MQ=255)                                                ggCCCACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccat                             <  2:894376/81‑1 (MQ=255)                                                    cACGGATACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccatccat                         >  1:894416/1‑81 (MQ=255)                                                         aTACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGAt                    <  2:894378/81‑1 (MQ=255)                                                         aTACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGAt                    <  2:894377/81‑1 (MQ=255)                                                         aTACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGAt                    <  1:894380/81‑1 (MQ=255)                                                         aTACTCTTTCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGAt                    <  1:894379/81‑1 (MQ=255)                                                                ttCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTa              >  1:894417/1‑80 (MQ=255)                                                                ttCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTa              >  1:894425/1‑80 (MQ=255)                                                                 tCACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTa              <  1:894381/79‑1 (MQ=255)                                                                  cACCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTacac           >  2:894431/1‑81 (MQ=255)                                                                   aCCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACAcc          <  2:894382/81‑1 (MQ=255)                                                                   aCCATTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACAcc          <  2:894383/81‑1 (MQ=255)                                                                      aTTTCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGcc       <  1:894384/81‑1 (MQ=255)                                                                        ttCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCAc     >  1:894441/1‑81 (MQ=255)                                                                        ttCACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCAc     >  2:894415/1‑81 (MQ=255)                                                                          cACCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGa   >  1:894424/1‑81 (MQ=255)                                                                           aCCTAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAt  <  1:894385/81‑1 (MQ=255)                                                                              |                                                                              GCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAT  >  NC_000913/4347669‑4347823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑