Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,109	G→A	V29I (GTT→ATT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,109	0	G	A	100.0%	101.0 / NA	29	V29I (GTT→ATT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/7);  total (22/7)

AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186                                                                           |                                                                              aaGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAccc                                                                         <  2:899647/81‑1 (MQ=255)        aaaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTc                                                                   >  1:899681/1‑80 (MQ=255)        aaaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTc                                                                   >  2:899715/1‑80 (MQ=255)          aaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCa                                                                 >  1:899679/1‑80 (MQ=255)          aaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCa                                                                 >  1:899701/1‑80 (MQ=255)             tCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAg                                                             >  1:899704/1‑81 (MQ=255)              cGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAgg                                                            >  1:899689/1‑81 (MQ=255)              cGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAgg                                                            >  2:899699/1‑81 (MQ=255)               ggTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGc                                                           <  1:899648/81‑1 (MQ=255)                  aaCGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCg                                                         >  2:899708/1‑80 (MQ=255)                   acgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTa                                                       <  2:899649/81‑1 (MQ=255)                    cgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTaa                                                      >  2:899668/1‑81 (MQ=255)                     gacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAAc                                                     <  1:899650/81‑1 (MQ=255)                           cGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGtt                                               >  2:899684/1‑81 (MQ=255)                            gtgtGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTc                                              >  2:899707/1‑81 (MQ=255)                             tgtgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCt                                             >  1:899694/1‑81 (MQ=255)                             tgtgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCt                                             <  2:899651/81‑1 (MQ=255)                               tgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTgg                                           <  2:899652/81‑1 (MQ=255)                                      gctgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATc                                    >  2:899691/1‑81 (MQ=255)                                      gctgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATc                                    >  2:899705/1‑81 (MQ=255)                                         gcgcGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCttt                                 >  2:899700/1‑81 (MQ=255)                                          cgcgGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTc                                >  1:899697/1‑81 (MQ=255)                                             ggcgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGt                             >  1:899706/1‑81 (MQ=255)                                               cgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCTGTAACGTAGTTCTGGATAAATCTTTCGGTg                            >  2:899711/1‑80 (MQ=255)                                                  aaaCGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCAcc                        >  2:899698/1‑81 (MQ=255)                                                    aCGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGa                      >  1:899688/1‑81 (MQ=255)                                                      gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                     >  1:899695/1‑80 (MQ=255)                                                        aCTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCAt                  >  2:899709/1‑81 (MQ=255)                                                         cTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATc                 <  2:899653/81‑1 (MQ=255)                                                                        aaaaTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGtt  >  2:899716/1‑81 (MQ=255)                                                                           |                                                                              AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑