Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	423,601	A→G	K362E (AAA→GAA)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,601	0	A	G	100.0%	85.5 / NA	25	K362E (AAA→GAA)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/8);  total (17/8)

TGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGG  >  NC_000913/423525‑423676                                                                             |                                                                            tgtgGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCggaaga                                                                         <  2:95169/81‑1 (MQ=255)   tggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCggaagaaa                                                                       <  1:95170/81‑1 (MQ=255)    ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAAc                                                                      >  1:95205/1‑81 (MQ=255)    ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAAc                                                                      >  1:95213/1‑81 (MQ=255)        cATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAg                                                                  <  2:95172/81‑1 (MQ=255)        cATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCAGCGGAAGAAACCCAg                                                                  <  2:95171/81‑1 (MQ=255)            tGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCgg                                                              <  1:95173/81‑1 (MQ=255)              cTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGaa                                                            <  2:95174/81‑1 (MQ=255)                      ggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTatat                                                    >  1:95201/1‑81 (MQ=255)                         gggTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGt                                                 >  2:95209/1‑81 (MQ=255)                          ggTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTc                                                >  1:95219/1‑81 (MQ=255)                           gTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCg                                               <  1:95175/81‑1 (MQ=255)                                    gcAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtt                                      >  1:95204/1‑81 (MQ=255)                                    gcAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtt                                      >  2:95208/1‑81 (MQ=255)                                      aataatATGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt                                    <  1:95176/81‑1 (MQ=255)                                         aataTGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                 >  2:95218/1‑81 (MQ=255)                                         aataTGCAGCACGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                 >  2:95215/1‑81 (MQ=255)                                                agcaCGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCAc                          >  1:95227/1‑81 (MQ=255)                                                   aCGTTGCCGGGATCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGc                       <  1:95177/81‑1 (MQ=255)                                                               tCACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAgg           >  1:95214/1‑81 (MQ=255)                                                                cACCGAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAATATTTTGGCGATGCACGGCAATGGTTACAGGc          >  2:95225/1‑81 (MQ=255)                                                                    gAAGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAt      >  2:95210/1‑81 (MQ=255)                                                                     aaGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATTCACGGCAATGGTTACAGGCCGAtg     >  1:95233/1‑81 (MQ=255)                                                                     aaGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg     <  2:95178/81‑1 (MQ=255)                                                                      aGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtgt    >  2:95217/1‑81 (MQ=255)                                                                      aGCGGCGGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtgt    >  2:95231/1‑81 (MQ=255)                                                                         ggcgGAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg  >  1:95211/1‑80 (MQ=255)                                                                             |                                                                            TGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGG  >  NC_000913/423525‑423676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑