| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,390,171 | T→C | N64D (AAC→GAC) | psd ← | phosphatidylserine decarboxylase proenzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,390,171 | 0 | T | C | 97.8% | 153.8 / NA | 45 | N64D (AAC→GAC) | psd | phosphatidylserine decarboxylase proenzyme |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (19/25); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATC > NC_000913/4390096‑4390246 | caggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAgg > 1:904651/1‑81 (MQ=255) aggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGt > 1:904655/1‑81 (MQ=255) aGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTg > 2:904650/1‑81 (MQ=255) tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGc < 1:904590/81‑1 (MQ=255) tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGc < 2:904589/81‑1 (MQ=255) cATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCgg > 1:904660/1‑81 (MQ=255) cGGATCGGTATCGATTGGGCGTACTTCGTCACGCGGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTc > 1:904639/1‑81 (MQ=255) cGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGgtgt < 1:904591/80‑1 (MQ=255) gATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCg < 1:904592/81‑1 (MQ=255) gATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCg > 1:904668/1‑81 (MQ=255) aTCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCgg < 2:904593/81‑1 (MQ=255) cGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCt > 2:904662/1‑81 (MQ=255) ggTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCtt > 1:904624/1‑81 (MQ=255) ggTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCtt > 2:904646/1‑81 (MQ=255) ggTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCtt < 2:904594/81‑1 (MQ=255) gTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCttt < 1:904595/81‑1 (MQ=255) aTCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTg > 2:904654/1‑81 (MQ=255) tCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTgc < 1:904596/81‑1 (MQ=255) tCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTg < 2:904597/80‑1 (MQ=255) cGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTgcg > 2:904652/1‑81 (MQ=255) cGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTgcg > 2:904628/1‑81 (MQ=255) gATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTgcg < 2:904598/80‑1 (MQ=255) ttGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCct > 2:904638/1‑81 (MQ=255) ggCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCtt < 2:904599/81‑1 (MQ=255) gCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCttt > 1:904666/1‑81 (MQ=255) cTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATg < 2:904600/80‑1 (MQ=255) cTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt > 1:904653/1‑81 (MQ=255) gTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAc > 1:904658/1‑81 (MQ=255) tCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAcc < 2:904601/81‑1 (MQ=255) gCAGCGGACGGACAAAGTATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgt < 2:904602/81‑1 (MQ=255) gCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgt < 1:904604/81‑1 (MQ=255) gCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgt < 1:904603/81‑1 (MQ=255) aGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgtag > 2:904661/1‑81 (MQ=255) gCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgtagt < 1:904605/81‑1 (MQ=255) ggacggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAt < 2:904606/81‑1 (MQ=255) ggacggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAt < 2:904607/81‑1 (MQ=255) ggacggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAt > 1:904667/1‑81 (MQ=255) gacggacAAAGAATTCGTCAAAGGTGCGGTAACTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAtt < 2:904608/81‑1 (MQ=255) acggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAttt < 2:904610/81‑1 (MQ=255) acggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAttt < 1:904609/81‑1 (MQ=255) acggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAtt > 1:904611/1‑80 (MQ=255) ggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTaa < 1:904612/81‑1 (MQ=255) gAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGaa > 1:904665/1‑78 (MQ=255) aaTTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGa < 2:904613/81‑1 (MQ=255) ttCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATc < 2:904614/81‑1 (MQ=255) | CAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATC > NC_000913/4390096‑4390246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |