Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,399,875	A→G	E208E (GAA→GAG)	miaA →	tRNA dimethylallyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,399,875	0	A	G	100.0%	143.5 / NA	42	E208E (GAA→GAG)	miaA	tRNA dimethylallyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

AACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTT  >  NC_000913/4399797‑4399951                                                                               |                                                                             aaCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCt                                                                            >  1:907229/1‑81 (MQ=255)  aCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCt                                                                            <  1:907169/80‑1 (MQ=255)  aCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTg                                                                           >  1:907230/1‑81 (MQ=255)   cGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGc                                                                          <  1:907170/81‑1 (MQ=255)    ggAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAgctgct                                                                         <  1:907171/81‑1 (MQ=255)       aCTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCa                                                                      >  1:907242/1‑81 (MQ=255)             gCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACg                                                                >  1:907217/1‑81 (MQ=255)                aaCGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGATCCGTGAGCTGCTCCATCAACGCAt                                                             >  2:907226/1‑81 (MQ=255)                 aCGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCAtt                                                            >  1:907215/1‑81 (MQ=255)                 aCGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCAtt                                                            >  2:907219/1‑81 (MQ=255)                  cGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTg                                                           <  2:907173/81‑1 (MQ=255)                  cGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTg                                                           <  1:907172/81‑1 (MQ=255)                     cAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGag                                                         >  1:907237/1‑80 (MQ=255)                       ggAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGagcag                                                      >  2:907224/1‑81 (MQ=255)                       ggAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGagcag                                                      <  2:907175/81‑1 (MQ=255)                         agaCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCg                                                    >  1:907214/1‑81 (MQ=255)                            cGCTATACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGttt                                                 <  2:907176/81‑1 (MQ=255)                                    cGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTtcat                                             >  2:907240/1‑77 (MQ=255)                                    cGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTtcat                                             >  1:907235/1‑77 (MQ=255)                                        tCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGtt                                     >  2:907220/1‑81 (MQ=255)                                               cATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTc                               <  1:907177/80‑1 (MQ=255)                                               cATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTc                               <  2:907178/80‑1 (MQ=255)                                               cATCAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTc                               <  2:907179/80‑1 (MQ=255)                                                  cAGTTCGCCATCTCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGt                           >  2:907222/1‑81 (MQ=255)                                                  cAGTTCGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGt                           >  1:907221/1‑81 (MQ=255)                                                       cGCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTg                       <  1:907180/80‑1 (MQ=255)                                                        gCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTg                       >  2:907257/1‑79 (MQ=255)                                                        gCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGaa                     <  2:907181/81‑1 (MQ=255)                                                          cATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGc                   <  2:907182/81‑1 (MQ=255)                                                          cATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGc                   <  1:907183/81‑1 (MQ=255)                                                           aTCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAg                    >  2:907228/1‑79 (MQ=255)                                                              gCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGa                <  2:907184/80‑1 (MQ=255)                                                              gCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGa                <  1:907185/80‑1 (MQ=255)                                                                 ccGGCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTc            <  1:907186/81‑1 (MQ=255)                                                                   ggCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCg          >  1:907227/1‑81 (MQ=255)                                                                   ggCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCg          >  1:907225/1‑81 (MQ=255)                                                                   ggCGAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCg          <  1:907187/81‑1 (MQ=255)                                                                      gAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgc       <  2:907188/81‑1 (MQ=255)                                                                      gAGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgc       <  2:907189/81‑1 (MQ=255)                                                                       aGCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgcg      <  1:907190/81‑1 (MQ=255)                                                                        gCCGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgcgc     >  2:907223/1‑81 (MQ=255)                                                                          cGTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCtt   <  2:907191/81‑1 (MQ=255)                                                                           gTGAGCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCttt  <  2:907192/81‑1 (MQ=255)                                                                               |                                                                             AACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTT  >  NC_000913/4399797‑4399951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑