Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,686	T→C	intergenic (+131/‑178)	fklB → / → cycA	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,686	0	T	C	100.0%	91.7 / NA	27	intergenic (+131/‑178)	fklB/cycA	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

CCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGC  >  NC_000913/4429609‑4429756                                                                              |                                                                       cccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCAt                                                                      >  2:915002/1‑80 (MQ=255)  ccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtcatca                                                                    <  1:914963/81‑1 (MQ=255)      cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCAcc                                                                  <  2:914964/79‑1 (MQ=255)       aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGt                                                                >  2:914996/1‑80 (MQ=255)       aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGt                                                                >  2:914992/1‑80 (MQ=255)       aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGt                                                                >  1:914999/1‑80 (MQ=255)         tttGCCAAACGGCGCTATTTTGTGTTGCAAAAACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGat                                                             <  2:914965/81‑1 (MQ=255)          ttGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGata                                                            >  2:915007/1‑81 (MQ=255)           tGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGatat                                                           >  2:915006/1‑81 (MQ=255)                aaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGacac                                                      <  2:914966/81‑1 (MQ=255)                   ggCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGAcactca                                                   >  1:915003/1‑81 (MQ=255)                      gcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACtt                                                >  1:914993/1‑81 (MQ=255)                       cTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACttt                                               <  2:914967/81‑1 (MQ=255)                         aTTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTa                                              >  1:915005/1‑80 (MQ=255)                          ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTaaa                                            <  1:914968/81‑1 (MQ=255)                             tgtgTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACa                                          <  1:914969/80‑1 (MQ=255)                                  tGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAAt                                    >  1:915010/1‑81 (MQ=255)                                       aGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa                               <  1:914970/81‑1 (MQ=255)                                        gACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt                              >  2:915009/1‑81 (MQ=255)                                           cccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAg                           <  1:914971/81‑1 (MQ=255)                                               tAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCt                       <  2:914972/81‑1 (MQ=255)                                                     tgtATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCt                 <  2:914973/81‑1 (MQ=255)                                                       tATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCt                 >  1:915004/1‑79 (MQ=255)                                                              gtgAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTc         <  2:914974/80‑1 (MQ=255)                                                              gtgAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTc         <  1:914975/80‑1 (MQ=255)                                                                  gCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATccc    <  2:914976/81‑1 (MQ=255)                                                                  gCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATccc    <  2:914977/81‑1 (MQ=255)                                                                      tttCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATAAACATTAGATCTAAATCTTAGTATTCATCCcgc  <  1:914978/79‑1 (MQ=255)                                                                              |                                                                       CCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGC  >  NC_000913/4429609‑4429756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑