Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,436,800	A→G	I16V (ATT→GTT)	cysQ →	3'(2'),5'‑bisphosphate nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,436,800	0	A	G	100.0%	175.4 / NA	50	I16V (ATT→GTT)	cysQ	3'(2'),5'‑bisphosphate nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

GTTAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGG  >  NC_000913/4436723‑4436875                                                                              |                                                                            gTTAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTa                                                                          <  2:916749/81‑1 (MQ=255)   tAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATg                                                                        >  1:916813/1‑81 (MQ=255)    aaCACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATg                                                                        >  1:916810/1‑80 (MQ=255)     acacCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGc                                                                       <  1:916750/80‑1 (MQ=255)      cacCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAg                                                                     >  1:916797/1‑81 (MQ=255)      cacCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAg                                                                     >  2:916815/1‑81 (MQ=255)         cGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTc                                                                  <  1:916751/81‑1 (MQ=255)          gCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTc                                                                  >  2:916817/1‑80 (MQ=255)          gCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTc                                                                  >  1:916814/1‑80 (MQ=255)           cTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTa                                                                <  2:916753/81‑1 (MQ=255)           cTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTa                                                                <  1:916752/81‑1 (MQ=255)              acaGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTacga                                                             <  2:916754/81‑1 (MQ=255)              acaGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTacg                                                              <  1:916755/80‑1 (MQ=255)                agagACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTacgacg                                                           <  2:916756/81‑1 (MQ=255)                agagACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTacgacg                                                           >  1:916804/1‑81 (MQ=255)                  agaCGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACggg                                                         <  2:916757/81‑1 (MQ=255)                  agaCGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACggg                                                         <  1:916758/81‑1 (MQ=255)                   gaCGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGa                                                        >  1:916809/1‑81 (MQ=255)                      gAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACg                                                      >  2:916826/1‑80 (MQ=255)                      gAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACg                                                      >  1:916839/1‑80 (MQ=255)                         gTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAAc                                                  <  2:916759/81‑1 (MQ=255)                           ggAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAAcc                                                 >  1:916830/1‑80 (MQ=255)                           ggAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAAcc                                                 >  1:916807/1‑80 (MQ=255)                             agaAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGAt                                              <  1:916760/81‑1 (MQ=255)                              gaAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATg                                             >  1:916825/1‑81 (MQ=255)                                 aTGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGAc                                          <  2:916761/81‑1 (MQ=255)                                 aTGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGAc                                          <  1:916762/81‑1 (MQ=255)                                    ttAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGAcgt                                        <  1:916763/80‑1 (MQ=255)                                       gATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGAcgtcgt                                     >  1:916818/1‑80 (MQ=255)                                          cAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAg                                  >  1:916824/1‑80 (MQ=255)                                           aaGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGc                                 >  2:916823/1‑80 (MQ=255)                                           aaGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGc                                 <  2:916764/80‑1 (MQ=255)                                           aaGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGc                                 >  2:916812/1‑80 (MQ=255)                                            aGTATGCCAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCa                                >  2:916845/1‑80 (MQ=255)                                                tGCCAGCTTGCACGGAATGCAGGCGATGCCGATATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCg                           <  2:916765/81‑1 (MQ=255)                                                  ccAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGa                         >  1:916822/1‑81 (MQ=255)                                                   cAGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGa                         >  2:916848/1‑80 (MQ=255)                                                    aGCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACa                       <  1:916766/81‑1 (MQ=255)                                                     gCTTGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACaa                      >  1:916832/1‑81 (MQ=255)                                                        tGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtc                   >  2:916803/1‑81 (MQ=255)                                                        tGCACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtc                   >  1:916829/1‑81 (MQ=255)                                                          cACGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtctc                 <  2:916767/81‑1 (MQ=255)                                                           aCGGAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTcc                >  2:916811/1‑81 (MQ=255)                                                              gAATGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCgg              >  1:916828/1‑80 (MQ=255)                                                               aaTGCAGGCGATGCCGTTATGCAGGTCTACGGCGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTa            <  2:916768/81‑1 (MQ=255)                                                               aaTGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGt             >  2:916816/1‑80 (MQ=255)                                                               aaTGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGt             >  1:916849/1‑80 (MQ=255)                                                               aaTGCAGGCGATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGt             >  2:916843/1‑80 (MQ=255)                                                                        gATGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCg   <  2:916769/81‑1 (MQ=255)                                                                         aTGCCGTTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCgg  <  1:916770/81‑1 (MQ=255)                                                                              |                                                                            GTTAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGG  >  NC_000913/4436723‑4436875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑