Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,477,066	G→A	V544I (GTT→ATT)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,477,066	0	G	A	100.0%	27.5 / NA	9	V544I (GTT→ATT)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/2);  total (7/2)

GATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTT  >  NC_000913/4476991‑4477140                                                                            |                                                                           gATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAAtattat                                                                        <  2:926621/80‑1 (MQ=255)            atatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACt                                                            >  2:926637/1‑81 (MQ=255)               tGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGa                                                          <  1:926622/80‑1 (MQ=255)                 aTTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGaat                                                        >  1:926638/1‑80 (MQ=255)                          ataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATAc                                               >  1:926636/1‑80 (MQ=255)                                   aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAt                                     <  1:926623/81‑1 (MQ=255)                                   aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAt                                     >  1:926634/1‑81 (MQ=255)                                                   aaTTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAAc                     >  2:926663/1‑81 (MQ=255)                                                       ttGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATc                 >  1:926635/1‑81 (MQ=255)                                                                     aGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGt   >  1:926645/1‑81 (MQ=255)                                                                       ataataTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGtt  >  1:926648/1‑80 (MQ=255)                                                                            |                                                                           GATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTT  >  NC_000913/4476991‑4477140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑