Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,241	G→A	L491L (TTG→TTA)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,241	0	G	A	100.0%	127.9 / NA	36	L491L (TTG→TTA)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

GCTGATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGT  >  NC_000913/49166‑49316                                                                            |                                                                            gCTGATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGat                                                                        >  1:11934/1‑81 (MQ=255)  cTGATTAACGCCATCGACGATCCGCAAACCAACATGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGatt                                                                       <  1:11892/81‑1 (MQ=255)           gCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgc                                                              <  1:11893/81‑1 (MQ=255)           gCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgc                                                              >  2:11943/1‑81 (MQ=255)            ccATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgcg                                                             >  1:11939/1‑81 (MQ=255)               tCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgcgccc                                                          <  2:11894/81‑1 (MQ=255)                 gacgaTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgcgcccgc                                                        <  1:11895/81‑1 (MQ=255)                 gacgaTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTgcccgcgcccgc                                                        >  1:11948/1‑81 (MQ=255)                    gaTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGAt                                                     <  2:11896/81‑1 (MQ=255)                    gaTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGAt                                                     >  1:11944/1‑81 (MQ=255)                     aTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATg                                                    <  1:11897/81‑1 (MQ=255)                     aTCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATg                                                    >  2:11940/1‑81 (MQ=255)                         gCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGa                                                >  1:11932/1‑81 (MQ=255)                         gCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGa                                                <  2:11898/81‑1 (MQ=255)                             accaaccTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCAc                                            <  2:11899/81‑1 (MQ=255)                               caaccTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTa                                          <  1:61898/81‑1 (MQ=255)                               caaccTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTa                                          <  2:11900/81‑1 (MQ=255)                                aaccTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTAc                                         <  2:11901/81‑1 (MQ=255)                                     gCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCg                                    >  2:11950/1‑81 (MQ=255)                                      cAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGt                                   >  2:11955/1‑81 (MQ=255)                                      cAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGt                                   <  2:11902/81‑1 (MQ=255)                                       aaCTGACAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGtt                                  <  2:11903/81‑1 (MQ=255)                                            aCAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGc                               <  1:11905/79‑1 (MQ=255)                                            aCAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGc                               <  1:11904/79‑1 (MQ=255)                                            aCAGAGATGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGc                               <  2:11906/79‑1 (MQ=255)                                               gagaTGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTcag                          >  2:11956/1‑81 (MQ=255)                                                agaTGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTcagg                         >  2:11949/1‑81 (MQ=255)                                                  aTGGTGAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTcaggca                       <  2:11907/81‑1 (MQ=255)                                                       gAAAGAACATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGt                  >  2:11946/1‑81 (MQ=255)                                                            aaCATTTCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGaa              >  1:11964/1‑80 (MQ=255)                                                                tttCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGcc         <  2:11908/81‑1 (MQ=255)                                                                  tCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCg        >  1:11970/1‑80 (MQ=255)                                                                  tCCCGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCg        >  2:11952/1‑80 (MQ=255)                                                                   cccGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCgg       <  1:11909/80‑1 (MQ=255)                                                                    ccGCATTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGa      <  1:11910/80‑1 (MQ=255)                                                                        aTTTACAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGt  >  1:11959/1‑80 (MQ=255)                                                                            |                                                                            GCTGATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTGACAGAGATGGTGAAAGAACATTTCCCGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGT  >  NC_000913/49166‑49316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑