Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,503,638	A→G	intergenic (‑207/‑420)	insG ← / → yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,503,638	0	A	G	100.0%	104.1 / NA	31	intergenic (‑207/‑420)	insG/yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/21);  total (10/21)

TTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGT  >  NC_000913/4503567‑4503713                                                                        |                                                                            tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATAc                                                                    <  1:932822/81‑1 (MQ=255)    gtgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAg                                                                  <  2:932823/80‑1 (MQ=255)         aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGc                                                            <  2:932824/81‑1 (MQ=255)           cAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCa                                                           >  2:932870/1‑80 (MQ=255)             gTAACAGAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAg                                                        <  2:932825/81‑1 (MQ=255)               aaCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGa                                                       >  1:932864/1‑80 (MQ=255)                aCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGaaa                                                     <  1:932826/81‑1 (MQ=255)                     aCGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTg                                                <  1:932827/81‑1 (MQ=255)                        cgTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCaa                                             <  2:932828/81‑1 (MQ=255)                          ttttGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAg                                           <  1:932829/81‑1 (MQ=255)                               ttATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaa                                       <  1:932831/80‑1 (MQ=255)                               ttATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaa                                       <  2:932830/80‑1 (MQ=255)                                  tgtgGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAg                                   >  2:932865/1‑81 (MQ=255)                                   gtgGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                  >  1:932869/1‑81 (MQ=255)                                   gtgGATTGCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                  >  1:932861/1‑81 (MQ=255)                                          gCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAAc                            <  1:932832/80‑1 (MQ=255)                                          gCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAAc                            <  2:932834/80‑1 (MQ=255)                                          gCTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAAc                            <  2:932833/80‑1 (MQ=255)                                           cTTGTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGt                          <  2:932835/81‑1 (MQ=255)                                              gTTTTTTGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTa                         <  1:932836/79‑1 (MQ=255)                                                tttttGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGc                      >  1:932868/1‑80 (MQ=255)                                                 ttttGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCt                     <  2:932837/80‑1 (MQ=255)                                                  tttGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTa                    >  2:932880/1‑80 (MQ=255)                                                  tttGATCTGGTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTa                    <  1:932838/80‑1 (MQ=255)                                                          ggTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaatag           >  2:932866/1‑80 (MQ=255)                                                          ggTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaatag           >  1:932875/1‑80 (MQ=255)                                                           gTATAACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaat             <  1:932839/78‑1 (MQ=255)                                                              taACAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCctc       <  1:932840/81‑1 (MQ=255)                                                                 cAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCctct      >  1:932867/1‑79 (MQ=255)                                                                 cAGGTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCa    <  2:932841/81‑1 (MQ=255)                                                                   ggTATGAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGt  <  1:932842/81‑1 (MQ=255)                                                                        |                                                                            TTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGT  >  NC_000913/4503567‑4503713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑