Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,865	A→G	G148G (GGT→GGC)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,865	0	A	G	100.0%	145.9 / NA	42	G148G (GGT→GGC)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/28);  total (14/28)

GGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTA  >  NC_000913/4513793‑4513941                                                                         |                                                                             gggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAt                                                                      >  1:935051/1‑81 (MQ=255)  ggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAtt                                                                     <  1:934991/81‑1 (MQ=255)  ggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAtt                                                                     <  1:934990/81‑1 (MQ=255)  ggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAt                                                                      <  1:934992/80‑1 (MQ=255)      gctggctgGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGc                                                                 <  2:934993/81‑1 (MQ=255)       ctggctggCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGcc                                                                <  1:934994/81‑1 (MQ=255)       ctggctggCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGcc                                                                >  1:935052/1‑81 (MQ=255)          gctggcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCgat                                                             <  1:934995/81‑1 (MQ=255)          gctggcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCgat                                                             <  1:934996/81‑1 (MQ=255)              gcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCgatgat                                                          <  1:934997/80‑1 (MQ=255)                ccACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGc                                                       <  2:934998/81‑1 (MQ=255)                 cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                      >  1:935050/1‑81 (MQ=255)                 cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                      <  2:934999/81‑1 (MQ=255)                 cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                      <  2:935001/81‑1 (MQ=255)                 cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                      <  1:935000/81‑1 (MQ=255)                  aCTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                     >  2:935057/1‑81 (MQ=255)                  aCTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCCCTCGTTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                     >  1:935067/1‑81 (MQ=255)                     gcgcCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                     >  2:935072/1‑78 (MQ=255)                       gcCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGAt                                                >  1:935063/1‑81 (MQ=255)                         cATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTg                                              <  1:935002/81‑1 (MQ=255)                         cATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTg                                              <  2:935003/81‑1 (MQ=255)                          aTCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGc                                             <  1:935004/81‑1 (MQ=255)                             ctctctTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCaaa                                          <  2:935005/81‑1 (MQ=255)                                tctTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAAttt                                       >  1:935087/1‑81 (MQ=255)                                    tATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTc                                     <  2:935007/79‑1 (MQ=255)                                    tATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCa                                    <  1:935006/80‑1 (MQ=255)                                          gttCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAg                              <  2:935008/80‑1 (MQ=255)                                             ccAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGtt                           <  1:935009/80‑1 (MQ=255)                                              cAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTc                         <  1:935010/81‑1 (MQ=255)                                              cAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTc                         <  2:935011/81‑1 (MQ=255)                                                gACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGt                       >  2:935065/1‑81 (MQ=255)                                                gACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGt                       <  2:935012/81‑1 (MQ=255)                                                       cTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGa                >  1:935104/1‑81 (MQ=255)                                                          cATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACtt             <  1:935014/81‑1 (MQ=255)                                                          cATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACtt             <  2:935015/81‑1 (MQ=255)                                                          cATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACt              >  1:935062/1‑80 (MQ=255)                                                          cATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACt              <  2:935013/80‑1 (MQ=255)                                                              tctcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAag          >  1:935016/1‑80 (MQ=255)                                                                tcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTaa           <  2:935017/77‑1 (MQ=255)                                                                 cGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAagcag       >  2:935089/1‑80 (MQ=255)                                                                  gCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAgcagca     <  1:935018/81‑1 (MQ=255)                                                                     ttttGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTa  >  1:935077/1‑81 (MQ=255)                                                                         |                                                                             GGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTA  >  NC_000913/4513793‑4513941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑