Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,631	T→C	K4R (AAA→AGA)	cyoB ←	cytochrome bo3 ubiquinol oxidase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,631	0	T	C	100.0%	102.2 / NA	30	K4R (AAA→AGA)	cyoB	cytochrome bo3 ubiquinol oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

CCAGACCTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCAT  >  NC_000913/450554‑450704                                                                              |                                                                          ccAGACCTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTc                                                                        <  2:101129/81‑1 (MQ=255)  cAGACCTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTcc                                                                       <  1:101131/81‑1 (MQ=255)  cAGACCTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTcc                                                                       <  2:101130/81‑1 (MQ=255)      ccTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAAc                                                                   <  2:101133/81‑1 (MQ=255)      ccTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAAc                                                                   <  2:101132/81‑1 (MQ=255)        tCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACAt                                                                 >  2:101167/1‑81 (MQ=255)         cccAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATc                                                                >  1:101162/1‑81 (MQ=255)               ataatGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTAtt                                                          >  1:101157/1‑81 (MQ=255)               ataatGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTAtt                                                          >  1:101159/1‑81 (MQ=255)               ataatGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTAtt                                                          >  1:101173/1‑81 (MQ=255)                  atGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTAttctt                                                       <  1:101134/81‑1 (MQ=255)                     ccAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCt                                                    <  2:101135/81‑1 (MQ=255)                             cGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCt                                            >  2:101174/1‑81 (MQ=255)                              gTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCtt                                           >  1:101177/1‑81 (MQ=255)                              gTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCtt                                           >  2:101186/1‑81 (MQ=255)                                aaCCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCttt                                          >  1:101169/1‑80 (MQ=255)                                aaCCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCttt                                          >  1:101164/1‑80 (MQ=255)                                  ccATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAAt                                       >  2:101160/1‑81 (MQ=255)                                       aCGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCg                                  <  2:101136/81‑1 (MQ=255)                                            aGGTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTc                             >  1:101161/1‑81 (MQ=255)                                             ggTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTcc                            >  2:101171/1‑81 (MQ=255)                                              gTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCcg                           <  2:101137/81‑1 (MQ=255)                                              gTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCcg                           <  2:101138/81‑1 (MQ=255)                                              gTTCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCcg                           >  2:101158/1‑81 (MQ=255)                                               ttCATGGAACGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCcgc                          <  2:101139/81‑1 (MQ=255)                                                      aaCGGGACTGCATCAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTc                   <  2:101140/81‑1 (MQ=255)                                                                   cAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCtt      >  2:101176/1‑81 (MQ=255)                                                                   cAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCtt      >  1:101179/1‑81 (MQ=255)                                                                   cAAGTGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCtt      >  2:101194/1‑81 (MQ=255)                                                                       tGATAATCTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCAt  <  1:101141/81‑1 (MQ=255)                                                                              |                                                                          CCAGACCTCCCAAAATAATGCCAGCGATCGTAACCATGACGATAGGTTCATGGAACGGGACTGCATCAAGTGATAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCAT  >  NC_000913/450554‑450704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑