Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,520,100	A→G	I75I (ATT→ATC)	yjhU ←	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,100	0	A	G	100.0%	122.9 / NA	36	I75I (ATT→ATC)	yjhU	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

AGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGT  >  NC_000913/4520023‑4520177                                                                              |                                                                              aGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGAtt                                                                            <  2:936754/81‑1 (MQ=255)  gCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGAttt                                                                           >  2:936789/1‑81 (MQ=255)     ttGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGc                                                                         >  1:936799/1‑80 (MQ=255)       gTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCaa                                                                       <  2:936755/80‑1 (MQ=255)        tATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCaaa                                                                      <  1:936756/80‑1 (MQ=255)            atcaCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAagcag                                                                  <  1:936757/80‑1 (MQ=255)            atcaCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAagcag                                                                  <  1:936758/80‑1 (MQ=255)             tcaCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCg                                                                >  1:936796/1‑81 (MQ=255)              caCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGt                                                               >  2:936804/1‑81 (MQ=255)                      ccGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATagag                                                       >  2:936810/1‑81 (MQ=255)                          cATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCaa                                                   <  1:936759/81‑1 (MQ=255)                          cATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCa                                                    >  1:936801/1‑80 (MQ=255)                            tGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCa                                                    >  2:936800/1‑78 (MQ=255)                             gTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGa                                                >  1:936806/1‑81 (MQ=255)                              ttCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGAc                                               <  2:936760/81‑1 (MQ=255)                                  ccATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                            >  2:936807/1‑80 (MQ=255)                                  ccATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                            >  1:936811/1‑80 (MQ=255)                                   cATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTca                                          >  2:936797/1‑81 (MQ=255)                                   cATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTca                                          >  2:936798/1‑81 (MQ=255)                                             gatgatCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGtt                                <  2:936761/81‑1 (MQ=255)                                               tgatCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGa                              <  1:936762/81‑1 (MQ=255)                                                 atCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGat                             <  1:936763/80‑1 (MQ=255)                                                       ctctGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAc                       >  2:936808/1‑80 (MQ=255)                                                          tGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAcc                      <  2:936765/78‑1 (MQ=255)                                                          tGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCa                     <  1:936766/79‑1 (MQ=255)                                                            gagaTTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTcc                 <  1:936767/81‑1 (MQ=255)                                                            gagaTTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTc                  >  2:936814/1‑80 (MQ=255)                                                                  gAATTTATCGCGGATTTGCAAAGCGGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCAtttt            <  1:936768/80‑1 (MQ=255)                                                                  gAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCAtttt            <  1:936769/80‑1 (MQ=255)                                                                   aaTTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTcc          <  2:936770/81‑1 (MQ=255)                                                                    aTTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTccc         <  1:936771/81‑1 (MQ=255)                                                                     tttATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCg        <  1:936772/81‑1 (MQ=255)                                                                       tATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCggg      >  2:936805/1‑81 (MQ=255)                                                                        aTCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGc     >  2:936813/1‑81 (MQ=255)                                                                         tCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCt    >  1:936828/1‑81 (MQ=255)                                                                           gcgGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGt  >  2:936819/1‑81 (MQ=255)                                                                              |                                                                              AGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGT  >  NC_000913/4520023‑4520177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑