Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,531,506	A→C	V49G (GTC→GGC)	sgcX ←	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,531,506	0	A	C	100.0%	144.4 / NA	42	V49G (GTC→GGC)	sgcX	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/25);  total (17/25)

ATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAG  >  NC_000913/4531431‑4531581                                                                            |                                                                            aTAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACa                                                                        >  1:939606/1‑81 (MQ=255)    aaaCCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTc                                                                     >  1:939613/1‑81 (MQ=255)     aaCCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTcc                                                                    >  2:939584/1‑81 (MQ=255)          aCTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGc                                                               <  1:939554/81‑1 (MQ=255)          aCTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGc                                                               >  1:939619/1‑81 (MQ=255)              cATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCt                                                             <  1:939555/79‑1 (MQ=255)               aTCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGtc                                                          <  1:939556/81‑1 (MQ=255)               aTCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGt                                                           <  1:939557/80‑1 (MQ=255)                tCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGtct                                                         >  2:939617/1‑81 (MQ=255)                  cATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCg                                                       >  1:939611/1‑81 (MQ=255)                    tatGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGcc                                                     <  1:939558/81‑1 (MQ=255)                      tGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAg                                                   <  1:939559/81‑1 (MQ=255)                        cgcAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGa                                                  <  2:939560/80‑1 (MQ=255)                        cgcAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGAc                                                 <  2:939561/81‑1 (MQ=255)                          cAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACct                                               >  1:939592/1‑81 (MQ=255)                           aaaaaTCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACctc                                              >  2:939609/1‑81 (MQ=255)                            aaaaTCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACctct                                             <  1:939562/81‑1 (MQ=255)                             aaaTCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCtt                                            >  1:939634/1‑81 (MQ=255)                                tcatcaGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTg                                         <  1:939563/81‑1 (MQ=255)                                tcatcaGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTg                                         >  1:939605/1‑81 (MQ=255)                                 catcaGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGc                                        >  1:939615/1‑81 (MQ=255)                                  atcaGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGc                                        <  2:939564/80‑1 (MQ=255)                                      gTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGAc                                   <  1:939565/81‑1 (MQ=255)                                         gCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGcc                                >  2:939608/1‑81 (MQ=255)                                           aGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTg                              <  1:939567/81‑1 (MQ=255)                                           aGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTg                              <  2:939566/81‑1 (MQ=255)                                            gcgcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGa                             <  2:939568/81‑1 (MQ=255)                                              gcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAt                           >  1:939621/1‑81 (MQ=255)                                               cATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAtt                          <  2:939571/81‑1 (MQ=255)                                               cATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAtt                          <  1:939570/81‑1 (MQ=255)                                               cATCGGGTTTATCACTACCGTAGCGCGCGCCAACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAtt                          <  2:939569/81‑1 (MQ=255)                                                  cGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCg                        >  2:939640/1‑80 (MQ=255)                                                   gggTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGc                       <  2:939572/80‑1 (MQ=255)                                                    ggTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGcac                     <  1:939573/81‑1 (MQ=255)                                                    ggTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGcac                     <  2:939574/81‑1 (MQ=255)                                                         aTCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCAt                >  2:939624/1‑81 (MQ=255)                                                          tCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCAt                <  2:939575/80‑1 (MQ=255)                                                          tCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATg               <  1:939576/81‑1 (MQ=255)                                                              tACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGt           >  1:939618/1‑81 (MQ=255)                                                                ccGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTc          <  2:939577/80‑1 (MQ=255)                                                                   tAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCg      <  1:939578/81‑1 (MQ=255)                                                                    aGCGCGCGCCGACATTCCCAAGCCTGTCTAGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGa     >  2:939642/1‑81 (MQ=255)                                                                       gcgcgcCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAg  <  2:939579/81‑1 (MQ=255)                                                                            |                                                                            ATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAG  >  NC_000913/4531431‑4531581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑