BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,531,897	G→T	intergenic (‑246/+540)	sgcX ← / ← yjhP	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain/KpLE2 phage‑like element; putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,531,897	0	G	T	100.0%	95.8 / NA	27	intergenic (‑246/+540)	sgcX/yjhP	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain/KpLE2 phage‑like element; putative methyltransferase YjhP
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/13); total (14/13)

GGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAAT  >  NC_000913/4531820‑4531967

ggAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACg                                                                     >  2:939688/1‑81 (MQ=255)

ggAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACg                                                                     >  1:939693/1‑81 (MQ=255)

    agaCCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTcc                                                                 >  1:939695/1‑81 (MQ=255)

    agaCCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTcc                                                                 >  2:939685/1‑81 (MQ=255)

    agaCCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTcc                                                                 <  2:939643/81‑1 (MQ=255)

         aGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCtt                                                            <  2:939644/81‑1 (MQ=255)

          gTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTc                                                           <  1:939647/81‑1 (MQ=255)

          gTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTc                                                           <  2:939645/81‑1 (MQ=255)

          gTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTc                                                           <  1:939646/81‑1 (MQ=255)

            gATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCAt                                                         >  2:939698/1‑81 (MQ=255)

               tATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTAc                                                      >  1:939683/1‑81 (MQ=255)

                  ggTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTc                                                   >  1:939680/1‑81 (MQ=255)

                              gAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGa                                        >  2:939691/1‑80 (MQ=255)

                               aGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGAt                                       <  2:939648/80‑1 (MQ=255)

                                   tGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATAcccc                                  <  2:939649/81‑1 (MQ=255)

                                     aaTAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAg                                >  2:939681/1‑81 (MQ=255)

                                        aaaaCGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCT‑CCTCTTCATTACGTCCTGCACGTGGATACCCCAGCAtc                            >  2:939684/1‑81 (MQ=255)

                                          aaCGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCAtct                           <  2:939650/81‑1 (MQ=255)

                                           aCGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCAtctc                          >  1:939707/1‑81 (MQ=255)

                                             gcgGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCa                        >  2:939711/1‑81 (MQ=255)

                                               ggTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCAt                       >  2:939699/1‑80 (MQ=255)

                                                gTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCa                     <  2:939651/81‑1 (MQ=255)

                                                       ttATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGAt               <  1:939652/80‑1 (MQ=255)

                                                            gCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAg          <  2:939653/80‑1 (MQ=255)

                                                                cacTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGt     <  1:939654/81‑1 (MQ=255)

                                                                   tttGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAAt  >  2:939697/1‑81 (MQ=255)

                                                                     tgttgCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAAt  <  1:939655/79‑1 (MQ=255)

GGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAAT  >  NC_000913/4531820‑4531967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`