Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,731	A→G	V189A (GTA→GCA)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,731	0	A	G	100.0%	76.3 / NA	23	V189A (GTA→GCA)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

GATCTGCTTTCAGGAATGATGGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCA  >  NC_000913/4550654‑4550806                                                                              |                                                                            gATCTGCTTTCAGGAATGATGGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTgca                                                                           <  1:943581/80‑1 (MQ=255)          tCAGGAATGATGGTGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGAt                                                                   <  1:943582/79‑1 (MQ=255)            aGGAATGATGGTGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTc                                                               <  2:943585/81‑1 (MQ=255)            aGGAATGATGGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGt                                                                <  2:943583/80‑1 (MQ=255)            aGGAATGATGGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTc                                                               <  2:943584/81‑1 (MQ=255)                 tgatgGTGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAg                                                          <  2:943586/81‑1 (MQ=255)                    tgGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTAc                                                       <  1:943587/81‑1 (MQ=255)                        gTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCa                                                   >  1:943625/1‑81 (MQ=255)                         ttGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCAt                                                  <  2:943588/81‑1 (MQ=255)                              cgcTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGata                                             >  2:943628/1‑81 (MQ=255)                                    aGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAAcc                                       <  2:943589/81‑1 (MQ=255)                                          ccGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATccc                                  <  2:943590/80‑1 (MQ=255)                                            gAGGAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCat                               <  2:943591/81‑1 (MQ=255)                                               gAACTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATc                            <  1:943592/81‑1 (MQ=255)                                                  cTTCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCGTCCCCATATCcgc                         <  1:943593/81‑1 (MQ=255)                                                   ttCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCcgcg                        >  2:943640/1‑81 (MQ=255)                                                   ttCGGCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCcgcg                        >  1:943632/1‑81 (MQ=255)                                                      ggCAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCa                     >  1:943631/1‑81 (MQ=255)                                                        cAGGATCAGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCAt                    >  1:943623/1‑80 (MQ=255)                                                               aGACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTc            <  1:943594/81‑1 (MQ=255)                                                                gACCTGCGCAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCa           >  1:943637/1‑81 (MQ=255)                                                                  ccTGCGCAGATTGCACTTGGGATCGTCACCAATACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCAcc         <  2:943595/81‑1 (MQ=255)                                                                      cgcAGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAgcg     >  2:943641/1‑81 (MQ=255)                                                                         aGATTGCACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCa  >  2:943634/1‑81 (MQ=255)                                                                              |                                                                            GATCTGCTTTCAGGAATGATGGCGTTGGGCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCA  >  NC_000913/4550654‑4550806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑