Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,356	T→C	K308K (AAA→AAG)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,356	0	T	C	100.0%	135.0 / NA	39	K308K (AAA→AAG)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

GGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCC  >  NC_000913/4590278‑4590428                                                                               |                                                                         ggTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAtctt                                                                        >  2:952924/1‑81 (MQ=255)     atgaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGt                                                                    >  2:952938/1‑81 (MQ=255)     atgaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGt                                                                    >  2:952919/1‑81 (MQ=255)       gaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTc                                                                  >  2:952906/1‑81 (MQ=255)        aaCAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTcc                                                                 <  1:952872/81‑1 (MQ=255)        aaCAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTc                                                                  <  2:952871/80‑1 (MQ=255)        aaCAGGAACGGGAACAGAGCGCCTTTCCACAACGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTcc                                                                 <  2:952870/81‑1 (MQ=255)          cAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCgg                                                               >  1:952923/1‑81 (MQ=255)           aGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCggg                                                              <  1:952873/81‑1 (MQ=255)            ggAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGt                                                             <  1:952874/81‑1 (MQ=255)             gAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGtt                                                            <  1:952875/81‑1 (MQ=255)              aaCGGGAACAGAGCGCCTTCCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTc                                                           <  2:952876/81‑1 (MQ=255)                 gggAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAg                                                         >  1:952920/1‑80 (MQ=255)                      cAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGcaccac                                                   <  1:952877/81‑1 (MQ=255)                        gagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGa                                                 >  2:952934/1‑81 (MQ=255)                        gagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGa                                                 >  1:952932/1‑81 (MQ=255)                           cgcCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATAc                                              <  2:952878/81‑1 (MQ=255)                            gcCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATAc                                              >  1:952928/1‑80 (MQ=255)                                 tCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAgcg                                        >  2:952931/1‑81 (MQ=255)                                 tCCACAGCGGGCCAGTACCGTCAATGTACTAGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAgc                                         <  2:952879/80‑1 (MQ=255)                                   cacaGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGcc                                      <  2:952880/81‑1 (MQ=255)                                    acaGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAgcgc                                       >  1:952937/1‑79 (MQ=255)                                     caGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAg                                    >  1:952936/1‑81 (MQ=255)                                              aGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGAt                           <  2:952881/81‑1 (MQ=255)                                              aGTACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGAt                           <  1:952882/81‑1 (MQ=255)                                                tACCGTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATaa                         >  2:952925/1‑81 (MQ=255)                                                    gTCAATGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGcc                     >  1:952926/1‑81 (MQ=255)                                                       aaTGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGa                   <  1:952883/80‑1 (MQ=255)                                                        aTGTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAtt                 <  2:952884/81‑1 (MQ=255)                                                          gTACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAtttt               >  1:952943/1‑81 (MQ=255)                                                           tACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTc              <  1:952885/81‑1 (MQ=255)                                                           tACTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTc              <  2:952886/81‑1 (MQ=255)                                                             cTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAg            >  1:952941/1‑81 (MQ=255)                                                             cTGGGTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAg            <  2:952887/81‑1 (MQ=255)                                                               gggTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGTGCCAGGCCGACGATAACGCCGATTTTCAgg           >  2:952940/1‑80 (MQ=255)                                                                ggTCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGaa         <  1:952888/81‑1 (MQ=255)                                                                  tCATGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAgg       <  1:952889/81‑1 (MQ=255)                                                                     tGGCAGGCATCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTg    <  2:952890/81‑1 (MQ=255)                                                                       gcaggcaTCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGcc  <  2:952891/81‑1 (MQ=255)                                                                         aggcaTCTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGc   >  2:952955/1‑78 (MQ=255)                                                                               |                                                                         GGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCC  >  NC_000913/4590278‑4590428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑