| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,596,424 | T→C | I145I (ATT→ATC) | lgoD → | L‑galactonate oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,596,424 | 0 | T | C | 97.6% | 140.2 / ‑6.3 | 42 | I145I (ATT→ATC) | lgoD | L‑galactonate oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (24/17); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGT > NC_000913/4596349‑4596495 | tAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAAcc > 1:954290/1‑81 (MQ=255) tttGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTa < 1:954256/80‑1 (MQ=255) tttGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTAt > 2:954311/1‑81 (MQ=255) tGAGCGTGCTGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTAtt < 2:954257/80‑1 (MQ=255) tGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTAt > 1:954313/1‑79 (MQ=255) gAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAg > 1:954307/1‑81 (MQ=255) gAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAg > 1:954322/1‑81 (MQ=255) aGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAgc < 1:954258/81‑1 (MQ=255) gTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCg < 2:954259/81‑1 (MQ=255) gCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTg > 2:954333/1‑81 (MQ=255) gCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTg > 1:954330/1‑81 (MQ=255) gAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCg < 2:954261/81‑1 (MQ=255) gAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCg < 2:954260/81‑1 (MQ=255) aaCATTTTGCCCGCTGACGGTATTGACCCGCAGGCGGCAGCATTGATTGACCCTCTCGCTATTAGCGCCCATGCGGTgatc < 2:984804/81‑4 (MQ=255) aaCATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGt < 2:954262/81‑1 (MQ=255) aCATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTc > 1:954329/1‑81 (MQ=255) aCATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTc < 2:954263/81‑1 (MQ=255) aTTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTcgc < 2:954264/81‑1 (MQ=255) aTTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTcgc > 1:954337/1‑81 (MQ=255) gCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGc > 2:954316/1‑81 (MQ=255) cccGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGc > 1:954339/1‑80 (MQ=255) ccGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCa > 2:954338/1‑81 (MQ=255) gCAGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCAtt < 1:954265/81‑1 (MQ=255) aGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGc > 1:954314/1‑81 (MQ=255) aGACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGc > 1:954328/1‑81 (MQ=255) gACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCt > 1:954305/1‑81 (MQ=255) gACGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCt > 1:954325/1‑81 (MQ=255) aCGGTATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTc < 1:954266/81‑1 (MQ=255) tATTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCgg < 1:954267/81‑1 (MQ=255) aTTGACCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGc > 2:954327/1‑81 (MQ=255) aCCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAg < 1:954269/80‑1 (MQ=255) aCCCGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGc < 2:954268/81‑1 (MQ=255) cccGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCa < 2:954270/81‑1 (MQ=255) ccGCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAg > 2:954315/1‑81 (MQ=255) gCAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGt < 2:954271/81‑1 (MQ=255) cAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTg > 1:954344/1‑81 (MQ=255) cAGGCGGCAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTg > 1:954326/1‑81 (MQ=255) ggcggcAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCt > 1:954334/1‑81 (MQ=255) ggcAGCATTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCtggt > 1:954317/1‑81 (MQ=255) gcagcaTTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCtggtg > 2:954318/1‑81 (MQ=255) cagcaTTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCtggtgg < 2:954272/81‑1 (MQ=255) agcaTTGATCGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTggtggt > 1:954345/1‑81 (MQ=255) | TAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGT > NC_000913/4596349‑4596495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |