Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,605,539	T→G	E42A (GAG→GCG)	fhuF ←	hydroxamate siderophore iron reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,605,539	0	T	G	100.0%	160.1 / NA	47	E42A (GAG→GCG)	fhuF	hydroxamate siderophore iron reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/26);  total (21/26)

AACTTAGCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGC  >  NC_000913/4605462‑4605616                                                                              |                                                                              aaCTTAGCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCa                                                                            <  1:956399/81‑1 (MQ=255)   cTTAGCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGc                                                                          <  1:956400/81‑1 (MQ=255)      aGCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCa                                                                         <  1:956401/79‑1 (MQ=255)       gCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAAt                                                                      >  2:956458/1‑81 (MQ=255)              cGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCAc                                                                <  2:956402/80‑1 (MQ=255)                gTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGAt                                                             >  1:956446/1‑81 (MQ=255)                 tgatgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATg                                                            >  2:956461/1‑81 (MQ=255)                   atgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGtt                                                          <  1:956403/81‑1 (MQ=255)                   atgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGtt                                                          >  2:956451/1‑81 (MQ=255)                    tgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGttt                                                         <  1:956404/81‑1 (MQ=255)                      aCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGtttt                                                        <  1:956405/80‑1 (MQ=255)                      aCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGtttt                                                        <  2:956406/80‑1 (MQ=255)                        cATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCg                                                     <  1:956407/81‑1 (MQ=255)                        cATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCg                                                     <  2:956408/81‑1 (MQ=255)                          ttGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGAt                                                   <  2:956409/81‑1 (MQ=255)                            gcgcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGAtc                                                  <  1:956410/80‑1 (MQ=255)                             cgcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGAtcgt                                                <  1:956411/81‑1 (MQ=255)                              gcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGAtcgtc                                               >  1:956456/1‑81 (MQ=255)                               ccAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGAtcgtcg                                              >  1:956450/1‑81 (MQ=255)                                  gCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGccc                                           <  2:956412/81‑1 (MQ=255)                                   cGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGTTAAACGCCAGCAAATGTTTACGAAGTTTTTCGTTCGTCGCCCg                                          >  2:956466/1‑81 (MQ=255)                                    gTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCg                                          >  2:956469/1‑80 (MQ=255)                                    gTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGc                                         <  2:956413/81‑1 (MQ=255)                                       aTGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCAc                                       <  1:956414/80‑1 (MQ=255)                                        tGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACgg                                     >  2:956452/1‑81 (MQ=255)                                         ggCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGc                                    >  2:956448/1‑81 (MQ=255)                                          gCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCt                                   >  2:956454/1‑81 (MQ=255)                                           cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCtt                                  <  2:956415/81‑1 (MQ=255)                                             ttAAGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTgc                                >  2:956447/1‑81 (MQ=255)                                               aaGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTgcg                               <  1:956416/80‑1 (MQ=255)                                               aaGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTgcg                               >  1:956438/1‑80 (MQ=255)                                                aGTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCa                             <  1:956417/81‑1 (MQ=255)                                                 gTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTgcgc                              >  2:956465/1‑79 (MQ=255)                                                 gTGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTgcgc                              >  1:956464/1‑79 (MQ=255)                                                  tGGGGCAGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAg                           <  2:956418/81‑1 (MQ=255)                                                        aGGTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCAGCACGGCTTGCGCAAGCGt                        >  2:956484/1‑78 (MQ=255)                                                          gTTCATCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGAt                   <  2:956419/81‑1 (MQ=255)                                                              aTCCAGGCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTg               <  1:956420/81‑1 (MQ=255)                                                                   ggCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCt          <  2:956422/81‑1 (MQ=255)                                                                   ggCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCt          >  2:956459/1‑81 (MQ=255)                                                                   ggCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCt          <  1:956421/81‑1 (MQ=255)                                                                    gCGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTg         >  1:956468/1‑81 (MQ=255)                                                                     cGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTgg        <  2:956424/81‑1 (MQ=255)                                                                     cGGATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTgg        <  2:956423/81‑1 (MQ=255)                                                                       gATAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTg         >  1:956462/1‑78 (MQ=255)                                                                        aTAAACGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTg         >  1:956455/1‑77 (MQ=255)                                                                           aaCGCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGc  >  1:956472/1‑81 (MQ=255)                                                                              |                                                                              AACTTAGCACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGC  >  NC_000913/4605462‑4605616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑