Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,439	G→A	C35C (TGC→TGT)	yjjW ←	putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,439	0	G	A	100.0%	168.3 / NA	47	C35C (TGC→TGT)	yjjW	putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/19);  total (28/19)

GCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCT  >  NC_000913/4615362‑4615515                                                                              |                                                                             gCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAg                                                                           >  2:959025/1‑81 (MQ=255) gCCTGACGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAg                                                                           >  1:959039/1‑81 (MQ=255)         cGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGAtt                                                                   >  1:959049/1‑81 (MQ=255)          ggACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTg                                                                  >  1:959064/1‑81 (MQ=255)          ggACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTg                                                                  >  1:959048/1‑81 (MQ=255)          ggACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTg                                                                  >  2:959030/1‑81 (MQ=255)           gACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTg                                                                  >  2:959032/1‑80 (MQ=255)             cacTGTGGCACGCACTCCCCACAGTCATTGCATCGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAg                                                               >  2:959035/1‑81 (MQ=255)                 gtgGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCt                                                           <  2:958976/81‑1 (MQ=255)                  tgGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGccc                                                            <  2:958977/79‑1 (MQ=255)                    gcacgcacTCCCCACAGTTATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGc                                                         >  1:959085/1‑80 (MQ=255)                    gcacgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGc                                                         >  1:959033/1‑80 (MQ=255)                     cacgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCaa                                                       <  2:958978/81‑1 (MQ=255)                      acgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCaa                                                       >  1:959036/1‑80 (MQ=255)                       cgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCaaa                                                      >  1:959051/1‑80 (MQ=255)                        gcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCaaaaa                                                    >  2:959061/1‑81 (MQ=255)                          acTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCaaaaa                                                    >  1:959027/1‑79 (MQ=255)                          acTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACa                                                  <  1:958979/81‑1 (MQ=255)                           cTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACag                                                 <  2:958980/81‑1 (MQ=255)                              cccACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGc                                              >  2:959044/1‑81 (MQ=255)                               ccACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGcc                                             <  2:958981/81‑1 (MQ=255)                                cacaGTTATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCa                                            <  2:958982/81‑1 (MQ=255)                                 acaGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGc                                              >  2:959040/1‑78 (MQ=255)                                  caGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAga                                          <  1:958983/81‑1 (MQ=255)                                      cATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAgacgac                                      >  2:959029/1‑81 (MQ=255)                                         tGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGc                                   >  1:959057/1‑81 (MQ=255)                                           cAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCt                                 >  2:959045/1‑81 (MQ=255)                                           cAACGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCt                                 <  2:958984/81‑1 (MQ=255)                                            aaCGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTg                                <  2:958985/81‑1 (MQ=255)                                             aCGTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTgg                               <  1:958986/81‑1 (MQ=255)                                               gTCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGcc                             >  1:959043/1‑81 (MQ=255)                                                tCCCATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGAAAAAACAGAGACAGACGACTGCCTGGccc                            <  2:958987/81‑1 (MQ=255)                                                   cATCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTc                         <  1:958988/81‑1 (MQ=255)                                                    aTCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTc                         >  1:959070/1‑80 (MQ=255)                                                     tCGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCaa                       >  1:959073/1‑81 (MQ=255)                                                      cGTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAAc                      >  1:959059/1‑81 (MQ=255)                                                       gTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAAc                      <  2:958990/80‑1 (MQ=255)                                                       gTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAAc                      <  1:958989/80‑1 (MQ=255)                                                       gTCCACGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACg                     >  2:959050/1‑81 (MQ=255)                                                           aCGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAg                  >  1:959053/1‑80 (MQ=255)                                                            cGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGga                <  2:958991/81‑1 (MQ=255)                                                            cGGATTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGga                <  1:958992/81‑1 (MQ=255)                                                               aTTGTGACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGaa             <  1:958993/81‑1 (MQ=255)                                                                  gtgACAGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAggg          >  2:959058/1‑81 (MQ=255)                                                                       aGTTTTTACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATaa     >  1:959072/1‑81 (MQ=255)                                                                         tttttACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATc   >  2:959062/1‑81 (MQ=255)                                                                           tttACAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCt  <  2:958994/80‑1 (MQ=255)                                                                              |                                                                             GCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCT  >  NC_000913/4615362‑4615515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑