| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,639,493 | A→G | E439G (GAA→GGA) | creD → | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,639,493 | 0 | A | G | 96.9% | 102.6 / ‑5.0 | 32 | E439G (GAA→GGA) | creD | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (15/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTT > NC_000913/4639417‑4639569 | cTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGtt < 2:965198/81‑1 (MQ=255) tgatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACg < 1:965199/81‑1 (MQ=255) gTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGAt > 2:965242/1‑79 (MQ=255) gTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGAt > 2:965239/1‑79 (MQ=255) gTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCt < 2:965200/81‑1 (MQ=255) tAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTg > 1:965245/1‑81 (MQ=255) atatCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTgg > 2:965244/1‑80 (MQ=255) atCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGaaa > 1:965232/1‑81 (MQ=255) tCGAATGGGAGGCGGTTTCACTGCCGAAAAGGAAAGAAAGGAAAGAAGTTACAACGGACGATGAGTTACGTAGCGGGAAAt < 2:1029661/81‑1 (MQ=255) gACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAAt > 2:965235/1‑79 (MQ=255) aCTGGTATGCGTTTTCACTGCCGAAAATGAAGGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAg < 2:965201/81‑1 (MQ=255) tGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGaaa < 2:965202/81‑1 (MQ=255) tGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTAGAAATAAGGTTGaaa < 1:965203/81‑1 (MQ=255) cGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGaaaa < 1:965204/80‑1 (MQ=255) cGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGaaaa < 2:965205/80‑1 (MQ=255) cGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGaaaa < 2:965206/80‑1 (MQ=255) tttCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATa > 2:965251/1‑80 (MQ=255) tCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaaa < 1:965207/80‑1 (MQ=255) tCACTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaaa < 1:965208/80‑1 (MQ=255) aCTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaaaaa < 1:965209/80‑1 (MQ=255) aCTGCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAAc > 2:965248/1‑81 (MQ=255) gCCGAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGgc > 1:965246/1‑81 (MQ=255) gAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGgcgc > 1:965227/1‑80 (MQ=255) gAAAATGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCt < 2:965210/81‑1 (MQ=255) aaaTGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTaa < 1:965211/81‑1 (MQ=255) aaaTGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTa > 2:965241/1‑80 (MQ=255) aaaTGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTa > 1:965243/1‑80 (MQ=255) aaTGAAAGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTaaa < 2:965212/81‑1 (MQ=255) aaGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgcg > 2:965247/1‑81 (MQ=255) aGCCAGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgcgc < 2:965213/81‑1 (MQ=255) aGTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtt > 1:965253/1‑81 (MQ=255) gTAAAGGAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGttt > 2:965250/1‑81 (MQ=255) aaggagTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGttttt < 2:965214/80‑1 (MQ=255) | CTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTT > NC_000913/4639417‑4639569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |