| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 480,275 | A→G | L12L (TTA→CTA) | hha ← | hemolysin expression modulating protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 480,275 | 0 | A | G | 95.8% | 73.5 / ‑3.7 | 24 | L12L (TTA→CTA) | hha | hemolysin expression modulating protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (10/13); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTC > NC_000913/480202‑480349 | gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCg > 2:107102/1‑81 (MQ=255) aTAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATc < 1:107073/79‑1 (MQ=255) tAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGt < 1:107074/81‑1 (MQ=255) aTTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGttt < 1:107075/81‑1 (MQ=255) gTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGt < 1:107076/81‑1 (MQ=255) gTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGt < 1:107077/81‑1 (MQ=255) atttatttTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGtt > 2:107105/1‑80 (MQ=255) tctcGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGttttt > 1:107100/1‑81 (MQ=255) gATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTc < 2:107078/78‑1 (MQ=255) aTAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGa > 2:107101/1‑80 (MQ=255) aTAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGAc < 2:107079/81‑1 (MQ=255) acGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAACCGGTTTTCGTTAAAGGTTTTTCGGACATACt > 2:107107/1‑81 (MQ=255) cAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTAc < 2:107080/80‑1 (MQ=255) tCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGtt > 1:107103/1‑81 (MQ=255) tCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGt < 2:107081/80‑1 (MQ=255) tGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGtaa > 2:107106/1‑80 (MQ=255) gTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGtaat < 2:107082/80‑1 (MQ=255) aaCGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTaa > 1:107126/1‑81 (MQ=255) aaCGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTaa > 2:107114/1‑81 (MQ=255) cgacgTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACa < 2:107084/81‑1 (MQ=255) cgacgTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACa < 1:107083/81‑1 (MQ=255) gacgTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACaa < 1:107085/81‑1 (MQ=255) aCGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAc > 2:107111/1‑81 (MQ=255) gCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTc < 2:107086/81‑1 (MQ=255) | GATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTC > NC_000913/480202‑480349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |